Xu Jiao, Chao Jung, Dai Yun-Tao, Zhu Chao, Li Qi, Takehisa Tomoda, Wang Dan-Dan, Chen Shi-Lin
Institute of Chinese Materia Medica, China Academy of Chinese Medicinal Sciences, Beijing 100700, China.
School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Zhongguo Zhong Yao Za Zhi. 2018 Mar;43(5):868-872. doi: 10.19540/j.cnki.cjcmm.20171023.008.
To establish the quality control methods for the standard decoction of Forsythiae Fructus. Twelve batches of representative Forsythiae Fructus were collected to prepare standard decoction of Forsythiae Fructus, and then the parameters such as extraction ratio, transfer rate of the index components and pH value of the solution were calculated to evaluate the stability of the process. The simultaneous determination method of target components and fingerprint method were established, and ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was used to identify the main common peaks in the fingerprint to clarify the main chemical constituents in the decoction. The similarities of the fingerprints of standard decoction of Forsythiae Fructus were more than 0.9. The average extraction ratio of the standard decoction of Forsythiae Fructus was (15.53±6.27)%, and the transfer rate of forsythiaside A was(38.0±10.2)%. The method for evaluating the quality of standard decoction of Forsythiae Fructus was presented, providing reference for the quality control of products stemmed from the water extract of Forsythiae Fructus, with high similarity and uniform quality.
建立连翘标准汤剂的质量控制方法。收集12批代表性连翘制备连翘标准汤剂，计算提取率、指标成分转移率、溶液pH值等参数，评价工艺稳定性。建立了目标成分同步测定法和指纹图谱法，采用超高效液相色谱-四极杆飞行时间质谱（UPLC-Q-TOF-MS）对指纹图谱中的主要共有峰进行鉴定，明确汤剂中的主要化学成分。连翘标准汤剂指纹图谱相似度均大于0.9。连翘标准汤剂平均提取率为（15.53±6.27）%，连翘苷A转移率为（38.0±10.2）%。提出了连翘标准汤剂质量评价方法，为连翘水提取物产品的质量控制提供参考，相似度高，质量均匀。
