与多发性硬化症相关的产气荚膜梭菌 ε 毒素的证据。
Evidence of Clostridium perfringens epsilon toxin associated with multiple sclerosis.
机构信息
College of Life and Environmental Sciences, University of Exeter, Exeter, UK.
Blizard Institute, Queen Mary University of London, London, UK.
出版信息
Mult Scler. 2019 Apr;25(5):653-660. doi: 10.1177/1352458518767327. Epub 2018 Apr 21.
BACKGROUND
It was recently reported that, using Western blotting, some multiple sclerosis (MS) patients in the United States had antibodies against epsilon toxin (Etx) from Clostridium perfringens, suggesting that the toxin may play a role in the disease.
OBJECTIVE
We investigated for serum antibodies against Etx in UK patients with clinically definite multiple sclerosis (CDMS) or presenting with clinically isolated syndrome (CIS) or optic neuritis (ON) and in age- and gender-matched controls.
METHODS
We tested sera from CDMS, CIS or ON patients or controls by Western blotting. We also tested CDMS sera for reactivity with linear overlapping peptides spanning the amino acid sequence (Pepscan) of Etx.
RESULTS
Using Western blotting, 24% of sera in the combined CDMS, CIS and ON groups ( n = 125) reacted with Etx. In the control group ( n = 125), 10% of the samples reacted. Using Pepscan, 33% of sera tested reacted with at least one peptide, whereas in the control group only 16% of sera reacted. Out of 61 samples, 21 (43%) were positive to one or other testing methodology. Three samples were positive by Western blotting and Pepscan.
CONCLUSION
Our results broadly support the previous findings and the role of Etx in the aetiology of MS warrants further investigation.
背景
最近有报道称，使用 Western blot 技术，一些美国多发性硬化症（MS）患者的血清中存在针对梭状芽孢杆菌 ε 毒素（Etx）的抗体，这表明该毒素可能在疾病中起作用。
目的
我们检测了英国临床确诊多发性硬化症（CDMS）患者、出现临床孤立综合征（CIS）或视神经炎（ON）的患者以及年龄和性别匹配的对照组患者的血清中是否存在针对 Etx 的抗体。
方法
我们通过 Western blot 技术检测了 CDMS、CIS 或 ON 患者或对照组的血清。我们还检测了 CDMS 血清与 Etx 氨基酸序列的线性重叠肽（Pepscan）的反应性。
结果
使用 Western blot 技术，联合 CDMS、CIS 和 ON 组（n=125）的 24%的血清与 Etx 发生反应。在对照组（n=125）中，有 10%的样本发生反应。使用 Pepscan，33%的测试血清与至少一种肽发生反应，而在对照组中只有 16%的血清发生反应。在 61 个样本中，有 21 个（43%）对一种或另一种检测方法呈阳性。有 3 个样本同时通过 Western blot 和 Pepscan 检测呈阳性。
结论
我们的结果大体上支持之前的发现，Etx 在 MS 发病机制中的作用值得进一步研究。
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