School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, 211800, China; Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture, Nanjing Tech University, Nanjing, 211800, China.
School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, 211800, China.
Chemosphere. 2018 Aug;205:62-70. doi: 10.1016/j.chemosphere.2018.04.010. Epub 2018 Apr 6.
Zinc pyrithione (ZPT) is widely used in industrial and human daily life, due to its broad antimicrobial spectrum activity. Persistent accumulation of ZTP in the aquatic environment and bioaccumulation in the living organisms attracts more and more attention. However, only very limited information is available so far for the evaluation of systematic toxicity effects of ZPT on multiple organs development. This study intends to deepen our knowledge about the potential toxicity elicited by ZPT by assessing its acute effects on zebrafish (Danio rerio) through morphological, histological and molecular investigations. It has been verified that ZPT exhibits a broad spectrum of toxicity which causes growth retardation and tissue pathological and physiology alternations in heart, liver, eye, notochord, kidney and other organisms of zebrafish. The acute toxicity values of LC50 (95% CI) 96-h is calculated as 0.073 μM. Furthermore, the organ toxicity was verified due to up-regulation of expression of biomarker genes related to organ function and development. In sum, this study demonstrats systematic acute embryological and developmental toxicity of the ZPT on zebrafish embryos/larvae.
吡啶硫酮锌（ZPT）由于其广谱的抗菌活性，被广泛应用于工业和人类日常生活中。ZTP 在水环境中的持久性积累和在生物体内的生物累积引起了越来越多的关注。然而，目前对于 ZPT 对多种器官发育的系统毒性效应的评估，只有非常有限的信息。本研究旨在通过形态学、组织学和分子研究，深入了解 ZPT 潜在的毒性作用，评估其对斑马鱼（Danio rerio）的急性影响。已经证实，ZPT 具有广泛的毒性，导致斑马鱼的生长迟缓，以及心脏、肝脏、眼睛、脊索、肾脏和其他组织的组织病理学和生理学改变。96 小时的 LC50（95%置信区间）的急性毒性值计算为 0.073μM。此外，由于与器官功能和发育相关的生物标志物基因的表达上调，证实了器官毒性。总之，本研究证明了 ZPT 对斑马鱼胚胎/幼虫具有系统的急性胚胎发育毒性。
