University of Twente, Department of Tissue Regeneration, Enschede, 7500 AE, The Netherlands.
Faculty of Engineering, University of Oporto, 4200-465, Porto, Portugal.
Sci Rep. 2018 Apr 23;8(1):6386. doi: 10.1038/s41598-018-24667-7.
Biomaterial scaffolds that can form a template for tissue growth and repair forms the basis of many tissue engineering paradigms. Cell migration and colonisation is an important, and often overlooked, first step. In this study, fibrous guidance structures were produced via electrospinning and the effect of physical features such as fibre diameter (ranging from 500 nm to 10 μm) on endothelial cell migration was assessed. Using a modified wound healing assay, fibre diameter was found to have a significant effect on the rate of wound closure and the peak migration velocity of the cells with scaffold diameter shown to influence both morphology and alignment of the migrating cells. The expression, phosphorylation and distribution of focal adhesion kinase (FAK) was disrupted on the different scaffolds with small-diameter scaffolds exhibiting increased FAK phosphorylation with the kinase present in the cytosol whereas on large-diameter scaffolds FAK was largely restricted to focal adhesions at the cell periphery. This study demonstrates that electrospun scaffolds can be used to model cell migration on fibrous substrates, and particularly for the studying effects of physical features of the substrate, and that FAK is a key mediator of cell-scaffold interactions on migrating cells.
生物材料支架可以为组织生长和修复形成模板，这是许多组织工程范例的基础。细胞迁移和定植是一个重要的、常常被忽视的第一步。在这项研究中，通过静电纺丝生产了纤维状导向结构，并评估了纤维直径（从 500nm 到 10μm）等物理特征对内皮细胞迁移的影响。使用改良的划痕愈合实验发现，纤维直径对伤口闭合率有显著影响，支架直径对细胞的迁移速度和形态以及细胞的取向都有影响。在不同的支架上，粘着斑激酶（FAK）的表达、磷酸化和分布都被破坏了，小直径支架上的 FAK 磷酸化增加，激酶存在于细胞质中，而在大直径支架上，FAK 主要局限于细胞边缘的粘着斑。这项研究表明，静电纺丝支架可用于模拟纤维状基底上的细胞迁移，特别是用于研究基底物理特征的影响，并且粘着斑激酶（FAK）是迁移细胞与细胞-支架相互作用的关键介质。
