Ściskalska Milena, Milnerowicz Halina
Przegl Lek. 2016;73(10):690-3.
Tobacco smoke xenobiotics can damage lung epithelium causing the impairment of its functions and Clara cell destruction. It was shown that Clara cell secretory protein (CC16) can be recognized as a useful marker of toxic effect of tobacco smoke on lung epithelium and CC16 concentration in the blood was positively correlated with the continuity of blood-air barrier. The aim of our study was to examine whether tobacco smoke can cause changes in the concentration of cotinine and CC16 in initial smokers. The study was also aimed to assess weather serum CC16 concentration can be an early marker of tobacco smoke exposure.
The investigations were performed in the blood of 105 volunteers in 21-28 years old. The study population was divided into smokers and non-smokers on the base of personal interview and determination of cotinine concentration in serum (ELISA method). The concentration of Clara cells secretory protein (CC16) using commercial test (ELISA method) was measured.
It was shown a significant increase in cotinine concentration in smokers compared to nonsmokers in both women and men. An increase in CC16 concentration in the group of smoking women compared to non-smoking women was noted. The level of this protein in the blood of men was 33.67 ± 10.03 ng/ml and 32.96 ± 12.11 ng/ml in non-smokers and smokers, respectively. The analyze of CC16 concentration depending on the intensity of smoking was shown a significant influence of tobacco smoke exposure on CC16 level in the blood of women.
The observed changes in CC16 concentrations in terms of intensity of cigarette smoking can indicate a greater sensitivity female than men organism on tobacco smoke exposure. CC16 can be considered as a useful marker of tobacco smoke exposure in women.
烟草烟雾中的异生物质会损害肺上皮细胞，导致其功能受损和克拉拉细胞破坏。研究表明，克拉拉细胞分泌蛋白（CC16）可被视为烟草烟雾对肺上皮细胞毒性作用的有用标志物，血液中CC16浓度与血气屏障的连续性呈正相关。我们研究的目的是检验烟草烟雾是否会导致初吸烟者体内可替宁和CC16浓度发生变化。该研究还旨在评估血清CC16浓度是否可作为烟草烟雾暴露的早期标志物。
对105名年龄在21至28岁的志愿者的血液进行了调查。根据个人访谈和血清中可替宁浓度的测定（酶联免疫吸附测定法），将研究人群分为吸烟者和非吸烟者。使用商业检测方法（酶联免疫吸附测定法）测量克拉拉细胞分泌蛋白（CC16）的浓度。
结果显示，吸烟者的可替宁浓度与非吸烟者相比，无论男女均显著升高。与非吸烟女性相比，吸烟女性组的CC16浓度有所增加。男性血液中该蛋白的水平在非吸烟者和吸烟者中分别为33.67±10.03纳克/毫升和32.96±12.11纳克/毫升。根据吸烟强度对CC16浓度进行分析，结果表明烟草烟雾暴露对女性血液中CC16水平有显著影响。
观察到的CC16浓度随吸烟强度的变化表明，女性机体对烟草烟雾暴露的敏感性高于男性。CC16可被视为女性烟草烟雾暴露的有用标志物。
