Suppr超能文献

对一种处方素食饮食的聚合酶链反应分析及其在三只患有皮肤食物不良反应的犬中的应用。

PCR analysis of a prescription vegetarian diet and use in three dogs with cutaneous adverse food reactions.

作者信息

Aufox Erin E, May Elizabeth R, Frank Linda A, Kania Stephen A

机构信息

Department of Small Animal Clinical Sciences, University of Tennessee, 2407 River Drive, Knoxville, TN, 37996, USA.

Department of Biomedical and Diagnostic Sciences, University of Tennessee, Knoxville, TN, 37996, USA.

出版信息

Vet Dermatol. 2018 Apr 24. doi: 10.1111/vde.12545.

Abstract

BACKGROUND

Cutaneous adverse food reaction (CAFR) is diagnosed by performing an elimination diet trial utilizing prescription or home-cooked diets followed by provocative challenge.

OBJECTIVES

To report findings of PCR analysis of a prescription vegetarian diet (RCV) for undeclared proteins of animal origin, as well as to describe its utilization for diagnosis and management of dogs suspected of having CAFR.

ANIMALS

Three client-owned dogs.

METHODS

PCR analysis of RCV for 11 mammalian species and poultry. In three dogs, clinical examination, cytology, aerobic culture (if indicated) and at least one elimination diet trial with RCV.

RESULTS

In our case series, all dogs had a history of pruritus and recurrent pyoderma that resolved with infection control and an elimination diet trial. In cases 1 and 2, a diagnosis of CAFR was made following an elimination trial with RCV and provocative challenge. Case 3 had a previously confirmed diagnosis of CAFR and RCV was successfully used to maintain remission of CAFR-related signs. PCR testing of RCV was negative for 11 mammalian species and poultry.

CONCLUSIONS AND CLINICAL IMPORTANCE

The RCV diet was found not to contain any undeclared mammalian or avian proteins. In this case series, the RCV was successfully used to diagnose and maintain three dogs with CAFR.

摘要

背景

皮肤食物不良反应(CAFR)通过进行排除饮食试验来诊断,该试验使用处方饮食或家庭烹饪饮食,随后进行激发试验。

目的

报告对一种处方素食饮食(RCV)进行聚合酶链反应(PCR)分析以检测未申报的动物源性蛋白质的结果,并描述其在疑似患有CAFR的犬类诊断和管理中的应用。

动物

三只客户拥有的犬。

方法

对RCV进行针对11种哺乳动物和家禽的PCR分析。对三只犬进行临床检查、细胞学检查、需氧培养(如有指征)以及至少一次使用RCV的排除饮食试验。

结果

在我们的病例系列中,所有犬都有瘙痒和复发性脓皮病病史,通过感染控制和排除饮食试验后症状得到缓解。在病例1和病例2中,通过使用RCV进行排除试验和激发试验后诊断为CAFR。病例3之前已确诊为CAFR,RCV成功用于维持CAFR相关体征的缓解。RCV的PCR检测对11种哺乳动物和家禽均为阴性。

结论及临床意义

发现RCV饮食不含任何未申报的哺乳动物或禽类蛋白质。在这个病例系列中,RCV成功用于诊断和维持三只患有CAFR的犬的病情。

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验