Čulum Dušan, Čopra-Janićijević Amira, Vidic Danijela, Klepo Lejla, Tahirović Azra, Bašić Neđad, Maksimović Milka
Department of Chemistry, Faculty of Science, University of Sarajevo, Zmaja od Bosne 33-35, 71 000 Sarajevo, Bosnia and Herzegovina.
Department of Forest Ecology, Faculty of Forestry, University of Sarajevo, Zagrebačka 20, 71 000 Sarajevo, Bosnia and Herzegovina.
Foods. 2018 Apr 24;7(5):66. doi: 10.3390/foods7050066.
The aim of this work was the qualitative and quantitative determination of selected phenolic compounds in three species grown in Bosnia. plants are consumed for medicinal purposes and as foodstuff in the form of canned fruit, jam, jelly, tea, and wine. Two samples of plant material, dry leaves with flowers, and berries of three species— Gand., Gand., and Hegetschw.—were analyzed. Twelve ethanolic extracts were isolated from the selected plant material using Soxhlet and ultrasound extraction, respectively. Soxhlet extraction proved to be more effective than ultrasound extraction. A simple and sensitive method, high-performance liquid chromatography with electrochemical detection, HPLC-ED, was used for the simultaneous determination of phenolic acids and flavonoids in species. The content of gallic acid in the extracts ranged from 0.001 to 0.082 mg/g dry weight (DW), chlorogenic acid from 0.19 to 8.70 mg/g DW, and rutin from 0.03 to 13.49 mg/g DW. Two flavonoids, vitexin and hyperoside, commonly found in chemotaxonomic investigations of species, were not detected in the examined extracts. In general, leaves with flowers samples are richer in gallic acid and rutin, whereas the berries samples are richer in chlorogenic acid. Distinct similarities were found in the relative distribution of gallic acid among the three species. Extracts of had the highest content of all detected compounds, while significant differences were found in rutin content, depending on the plant organ. To the best of our knowledge, this is the first study reporting content of phenolic compounds in Gand., , and from Bosnia.
这项工作的目的是对波斯尼亚生长的三种植物中的选定酚类化合物进行定性和定量测定。这些植物被用于药用，也以罐装水果、果酱、果冻、茶和葡萄酒的形式作为食品。分析了三种植物（甘菊、德国洋甘菊和赫格施瓦尔德洋甘菊）的两个植物材料样本，即带花的干叶和浆果。分别使用索氏提取法和超声提取法从选定的植物材料中分离出12种乙醇提取物。结果表明，索氏提取法比超声提取法更有效。采用一种简单灵敏的方法——高效液相色谱 - 电化学检测法（HPLC - ED）同时测定这些植物中的酚酸和黄酮类化合物。提取物中没食子酸的含量范围为0.001至0.082毫克/克干重（DW），绿原酸为0.19至8.70毫克/克DW，芦丁为0.03至13.49毫克/克DW。在这些植物的化学分类研究中常见的两种黄酮类化合物，即牡荆素和金丝桃苷，在所检测的提取物中未被检测到。一般来说，带花的叶子样本中没食子酸和芦丁含量更高，而浆果样本中绿原酸含量更高。在这三种植物中没食子酸的相对分布上发现了明显的相似性。甘菊的提取物中所有检测到的化合物含量最高，并且根据植物器官的不同，芦丁含量存在显著差异。据我们所知，这是第一项报道波斯尼亚的甘菊、德国洋甘菊和赫格施瓦尔德洋甘菊中酚类化合物含量的研究。
