The 3rd Xiangya Hospital, Central South University, Changsha, China; School of Materials Science and Engineering, Central South University, Changsha, China.
State Key Laboratory of Medicinal Chemical Biology College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin, China; Dynamic Optical Microscopic Imaging Laboratory, Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Key Laboratory of Phytochemical R&D of Hunan Province, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, China.
Nanomedicine. 2018 Jul;14(5):1619-1628. doi: 10.1016/j.nano.2018.04.007. Epub 2018 Apr 23.
Keratinocyte growth factor (KGF) has been demonstrated to specifically stimulate the multiplication and migration of keratinocytes. However, due to rapid degradation, the results of topical application of growth factors on wounds are unsatisfactory. In this study, we cross-linked KGF to the surface of gold nanoparticles (GNPs) and explored their effects on wound healing. The as-synthesized nanocomposite (KGF-GNPs) displayed good colloidal stability, decent biocompatibility as well as negligible cellular cytotoxicity. The in vitro cellular experimental results demonstrated that KGF-GNPs could effectively promote the proliferation of keratinocytes in contrast to bare GNPs or KGF. Furthermore, in animal full-thickness wound model, KGF-GNPs are more conducive to wound healing than bare GNPs or KGF. KGF-GNPs enhanced wound healing by promoting wound re-epithelialization rather than granulation. The superior biocompatibility, colloidal depressiveness and biological activity of this nanocomposite indicate that it could be utilized as a promising wound healing drug for clinical application in the future.
角质细胞生长因子（KGF）已被证明能特异性刺激角质细胞的增殖和迁移。然而，由于生长因子的快速降解，其在伤口表面的应用效果并不理想。在这项研究中，我们将 KGF 交联到金纳米粒子（GNPs）的表面，并探索了它们对伤口愈合的影响。合成的纳米复合材料（KGF-GNPs）表现出良好的胶体稳定性、良好的生物相容性和可忽略的细胞毒性。体外细胞实验结果表明，与 bare GNPs 或 KGF 相比，KGF-GNPs 能有效促进角质细胞的增殖。此外，在动物全层伤口模型中，KGF-GNPs 比 bare GNPs 或 KGF 更有利于伤口愈合。KGF-GNPs 通过促进伤口再上皮化而不是肉芽形成来增强伤口愈合。这种纳米复合材料具有优越的生物相容性、胶体稳定性和生物活性，表明它有望成为未来临床应用中一种有前途的伤口愈合药物。
