Miller Jason R, Koren Sergey, Dilley Kari A, Harkins Derek M, Stockwell Timothy B, Shabman Reed S, Sutton Granger G
J. Craig Venter Institute, Rockville, MD, 20850, USA.
Shepherd University, Shepherdstown, WV, 25443, USA.
F1000Res. 2018 Mar 8;7:297. doi: 10.12688/f1000research.13635.1. eCollection 2018.
The tick cell line ISE6, derived from , is commonly used for amplification and detection of arboviruses in environmental or clinical samples. To assist with sequence-based assays, we sequenced the ISE6 genome with single-molecule, long-read technology. The draft assembly appears near complete based on gene content analysis, though it appears to lack some instances of repeats in this highly repetitive genome. The assembly appears to have separated the haplotypes at many loci. DNA short read pairs, used for validation only, mapped to the cell line assembly at a higher rate than they mapped to the reference genome sequence. The assembly could be useful for filtering host genome sequence from sequence data obtained from cells infected with pathogens.
蜱细胞系ISE6源自，常用于环境或临床样本中虫媒病毒的扩增和检测。为辅助基于序列的检测，我们使用单分子长读长技术对ISE6基因组进行了测序。基于基因含量分析，草图组装似乎已接近完成，不过在这个高度重复的基因组中，它似乎缺少一些重复序列实例。该组装在许多位点似乎已将单倍型分开。仅用于验证的DNA短读长对，比对到该细胞系组装的比例高于比对到参考基因组序列的比例。该组装对于从感染病原体的细胞获得的序列数据中过滤宿主基因组序列可能有用。
