Inhibition of using Bacteriophages and Bacteriocin Producing Strains.

In this study, we isolated and characterized a bacteriocin-producing strain and two bacteriophages (P4, A3), showing antimicrobial effects against , from chicken and swine feces by the spot-on-the lawn antagonism method. The selected strain was identified as by 16S rRNA gene sequencing. The bacteriocin from the isolated strain exhibited strong inhibitory activity against four strains of and all the tested strains of , and the bacteriocin were highly heat- and pH-stable even at pH 2, pH 10 and 121℃ for 15 min. We also evaluated the combined effects of the isolated bacteriocin and phages. Combining the phage treatments and bacteriocin resulted in a synergetic effect compared with the phage or the bacteriocin alone. In addition, during the probiotic test, the bacteriocin-producing B19 strain reduced the population of significantly. Treatment with B19 and a cocktail of lytic bacteriophages eradicated the KCTC 3269, completely. Consequently, the isolated bacteriocin and bacteriophages represent candidates for effective biocontrol of , and bacteriocin-producing B19 is a potential probiotic candidate for use in domestic animals.