Aghbali Amir Ala, Akbarzadeh Ayshin, Kouhsoltani Maryam
Department of Oral and Maxillofacial Pathology, Research Center of Biotechnology, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Prosthodontics, Drug Applied Research Center, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
J Oral Maxillofac Pathol. 2018 Jan-Apr;22(1):147. doi: 10.4103/jomfp.JOMFP_15_16.
Many studies have reported that macrophages and eosinophils are involved in the pathogenesis of several diseases. To the best of our knowledge, this is the first study comparing macrophages and eosinophils in oral reactive lesions.
In this study, we aimed to determine the contribution of macrophages and eosinophils to the pathogenesis of oral reactive lesions and the relationships between these biomarkers and the diverse histopathologic features.
Seventy-five paraffin-embedded tissue samples were assessed in this study. Five categories (15 cases for each group), including peripheral ossifying fibroma, pyogenic granuloma, fibroma, inflammatory fibrous hyperplasia, and peripheral giant-cell granuloma, were considered. Anti-CD68 immunohistochemical and hematoxylin-eosin staining was carried out.
We found that macrophages, but not eosinophils, were a significant internal component of oral reactive lesions. Macrophages were observed in high densities in all studied groups and diffusely distributed or clustered throughout these lesions. The number of macrophages was increased in peripheral giant-cell granuloma compared with other groups.
Our findings suggest that macrophages are involved in the pathogenesis and the variation of microscopic features of oral reactive lesions. However, further clinical studies should be conducted to identify the biological process behind macrophages and the molecular interactions of these cells, with the ultimate aim of suggesting a new potential therapeutic target for these lesions. We found that eosinophils were not involved in the fibrotic process and the variation of microscopic features in oral reactive lesions. Our results showed that peripheral giant-cell granulomas highly demonstrated histiocytic characteristics.
许多研究报告称巨噬细胞和嗜酸性粒细胞参与了多种疾病的发病机制。据我们所知，这是第一项比较口腔反应性病变中巨噬细胞和嗜酸性粒细胞的研究。
在本研究中，我们旨在确定巨噬细胞和嗜酸性粒细胞在口腔反应性病变发病机制中的作用，以及这些生物标志物与不同组织病理学特征之间的关系。
本研究评估了75个石蜡包埋的组织样本。考虑了五类（每组15例），包括外周骨化性纤维瘤、化脓性肉芽肿、纤维瘤、炎性纤维增生和外周巨细胞肉芽肿。进行了抗CD68免疫组织化学和苏木精-伊红染色。
我们发现巨噬细胞而非嗜酸性粒细胞是口腔反应性病变的重要内在组成部分。在所有研究组中均观察到高密度的巨噬细胞，它们在这些病变中弥漫分布或聚集。与其他组相比，外周巨细胞肉芽肿中巨噬细胞的数量增加。
我们的研究结果表明巨噬细胞参与了口腔反应性病变的发病机制和微观特征的变化。然而，应进行进一步的临床研究以确定巨噬细胞背后的生物学过程以及这些细胞的分子相互作用，最终目的是为这些病变提出新的潜在治疗靶点。我们发现嗜酸性粒细胞不参与口腔反应性病变的纤维化过程和微观特征的变化。我们的结果表明外周巨细胞肉芽肿高度显示组织细胞特征。
