Chakraborty Imon, Hossain Chowdhury Mobaswar, Basak Piyali
School of Bioscience and Engineering, Jadavpur University, Kolkata, India.
Maulana Abul Kalam Azad University of Technology, Salt Lake, Kolkata, West Bengal India.
Biomed Eng Lett. 2020 Nov 28;11(1):25-37. doi: 10.1007/s13534-020-00180-7. eCollection 2021 Feb.
Hypopharyngeal tissue engineering is increasing rapidly in this developing world. Tissue damage or loss needs the replacement by another biological or synthesized membrane using tissue engineering. Tissue engineering research is emerging to provide an effective solution for damaged tissue replacement. Polyurethane in tissue engineering has successfully been used to repair and restore the function of damaged tissues. In this context, Can polyurethane be a useful material to deal with hypopharyngeal tissue defects? To explore this, here ester diol based polyurethane (PU) was synthesized in two steps: firstly, polyethylene glycol 400 (PEG 400) was reacted with lactic acid to prepare ester diol, and then it was polymerized with hexamethylene diisocyanate. The physical, mechanical, and biological testing was done to testify the characterization of the membrane. The morphology of the synthesized membrane was investigated by using field emission scanning electron microscopy. Functional groups of the obtained membrane were characterized by fourier transform infrared spectroscopy spectroscopy. Several tests were performed to check the in vitro and in vivo biocompatibility of the membrane. A highly connected homogeneous network was obtained due to the appropriate orientation of a hard segment and soft segment in the synthesized membrane. Mechanical property analysis indicates the membrane has a strength of 5.15 MPa and strain 124%. The membrane showed high hemocompatibility, no cytotoxicity on peripheral blood mononuclear cell, and susceptible to degradation in simulated body fluid solution. Antimicrobial activity assessment has shown promising results against clinically significant bacteria. Primary hypopharyngeal cell growth on the PU membrane revealed the cytocompatibility and subcutaneous implantation on the back of Wistar rats were given in vivo biocompatibility of the membrane. Therefore, the synthesized material can be considered as a potential candidate for a hypopharyngeal tissue engineering application.
在这个发展中的世界里，下咽组织工程正在迅速发展。组织损伤或缺失需要使用组织工程用另一种生物或合成膜进行替代。组织工程研究正在兴起，为受损组织替代提供有效的解决方案。聚氨酯在组织工程中已成功用于修复和恢复受损组织的功能。在这种情况下，聚氨酯能否成为处理下咽组织缺损的有用材料？为了探究这一点，在此分两步合成了基于酯二醇的聚氨酯（PU）：首先，聚乙二醇400（PEG 400）与乳酸反应制备酯二醇，然后将其与六亚甲基二异氰酸酯聚合。进行了物理、机械和生物学测试以验证该膜的特性。使用场发射扫描电子显微镜研究了合成膜的形态。通过傅里叶变换红外光谱对所得膜的官能团进行了表征。进行了多项测试以检查该膜的体外和体内生物相容性。由于合成膜中硬段和软段的适当取向，获得了高度连通的均匀网络。力学性能分析表明该膜的强度为5.15 MPa，应变率为124%。该膜表现出高血液相容性，对外周血单核细胞无细胞毒性，并且在模拟体液溶液中易于降解。抗菌活性评估对临床上重要的细菌显示出有希望的结果。PU膜上的原代下咽细胞生长显示了细胞相容性，在Wistar大鼠背部的皮下植入给出了该膜的体内生物相容性。因此，合成材料可被视为下咽组织工程应用的潜在候选材料。
