Abdel Gawad Samah S, Ismail Mousa A M, Imam Naglaa F A, Eassa Ahmed H A, Abu-Sarea Enas Yahia
Parasitology Department, Faculty of Medicine, Beni-Suef University, Egypt.
Parasitology Department, Faculty of Medicine, Cairo University, Egypt.
Korean J Parasitol. 2018 Apr;56(2):113-119. doi: 10.3347/kjp.2018.56.2.113. Epub 2018 Apr 30.
species is an important cause of gastrointestinal infections globally. This study aimed to shed light on its role in diarrheic immunocompetent patients in Beni-Suef, Egypt and to compare three diagnostic methods. Two hundred diarrheic patients, 37±16.8 year old, were enrolled. Stool samples were examined by light microscopy, using modified Ziehl-Neelsen stain (MZN) for spp. oocysts. Coproantigens were detected by sandwich ELISA. DNA molecular diagnosis was done by nested PCR. PCR yielded the highest detection rates (21.0%), compared to ELISA (12.5%) and MZN staining method (9.5%). The higher infection rates were in 20-40 year-old group, followed by 40-60 year-old. Association between epidemiologic factors was statistically not significant; positivity and gender, clinical manifestations, residence, source or water, or contact with animals. Cryptosporidiosis is an important enteric parasitic infection in Beni-Suef and PCR remains the gold standard for diagnosis.
该物种是全球胃肠道感染的重要原因。本研究旨在阐明其在埃及贝尼苏韦夫腹泻免疫功能正常患者中的作用，并比较三种诊断方法。招募了200名年龄为37±16.8岁的腹泻患者。粪便样本通过光学显微镜检查，使用改良齐尔-尼尔森染色法（MZN）检测该物种的卵囊。通过夹心ELISA检测粪便抗原。DNA分子诊断通过巢式PCR进行。与ELISA（12.5%）和MZN染色法（9.5%）相比，PCR检测率最高（21.0%）。感染率较高的是20-40岁组，其次是40-60岁组。流行病学因素之间的关联在统计学上不显著；阳性与性别、临床表现、居住地、水源或与动物接触之间无关联。隐孢子虫病是贝尼苏韦夫一种重要的肠道寄生虫感染，PCR仍然是诊断的金标准。
