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免疫功能低下患者粪便样本中的检测。 （你提供的原文似乎不完整，“Detection of”后面缺少具体内容）

Detection of in stool samples of immunocompromised patients.

作者信息

Vanathy K, Parija Subhash Chandra, Mandal Jharna, Hamide Abdoul, Krishnamurthy Sriram

机构信息

Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India.

Department of Medicine, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India.

出版信息

Trop Parasitol. 2017 Jan-Jun;7(1):41-46. doi: 10.4103/tp.TP_66_16.

DOI:10.4103/tp.TP_66_16
PMID:28459014
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5369273/
Abstract

INTRODUCTION

species is the most common opportunistic enteric parasite encountered in the immunocompromised patients. Considering the need to diagnose them early relies mostly on rapid tests such as antigen detection by immunochromatographic test (ICT), ELISA, and microscopy. However, the sensitivity and specificity varies with different methods and different kits used. This study was conducted to determine the intestinal parasitic profile in immunocompromised patients and to assess the diagnostic accuracy of the ICT using ImmunoCard STAT kit in detecting spp.

MATERIALS AND METHODS

The patients in this study were divided into two groups: one group was immunocompromised patients ( = 73) and the other was nonimmunocompromised individuals ( = 73). Stool microscopy, ICT, and polymerase chain reaction (PCR) were carried out for all stool samples.

RESULTS

Totally, 4 (5.4%) of 73 patients of the study group were positive for . The species detected were and . PCR was taken as gold standard in the current study. PCR detected in four samples while ICT in two samples and microscopy in one sample.

CONCLUSION

was found to be the most common enteric parasite in the immunocompromised patients studied, followed by , , and . Although the ICT is a rapid test, it was less sensitive and more expensive in comparison to the PCR; hence, its utility appears to be limited in our setting.

摘要

引言

[寄生虫名称]是免疫功能低下患者中最常见的机会性肠道寄生虫。鉴于早期诊断的需要主要依赖于快速检测，如免疫层析试验（ICT）、酶联免疫吸附测定（ELISA）和显微镜检查等抗原检测方法。然而，不同方法和使用的不同试剂盒，其敏感性和特异性各不相同。本研究旨在确定免疫功能低下患者的肠道寄生虫谱，并评估使用免疫卡 STAT 试剂盒的 ICT 在检测[寄生虫名称]方面的诊断准确性。

材料与方法

本研究中的患者分为两组：一组是免疫功能低下患者（n = 73），另一组是免疫功能正常个体（n = 73）。对所有粪便样本进行粪便显微镜检查、ICT 和聚合酶链反应（PCR）。

结果

研究组的 73 名患者中，共有 4 例（5.4%）[寄生虫名称]检测呈阳性。检测到的[寄生虫名称]种类为[具体种类 1]和[具体种类 2]。在本研究中，PCR 被用作金标准。PCR 在四个样本中检测到[寄生虫名称]，而 ICT 在两个样本中检测到，显微镜检查在一个样本中检测到。

结论

在所研究的免疫功能低下患者中，[寄生虫名称]被发现是最常见的肠道寄生虫，其次是[其他寄生虫名称 1]、[其他寄生虫名称 2]和[其他寄生虫名称 3]。尽管 ICT 是一种快速检测方法，但与 PCR 相比，其敏感性较低且成本较高；因此，在我们的研究环境中，其应用似乎有限。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d55/5369273/88938a2ec375/TP-7-41-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d55/5369273/8eaf645d48be/TP-7-41-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d55/5369273/adbef369c468/TP-7-41-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d55/5369273/e5fe1ba51081/TP-7-41-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d55/5369273/34eb5daaf894/TP-7-41-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d55/5369273/d29e3406f73d/TP-7-41-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d55/5369273/88938a2ec375/TP-7-41-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d55/5369273/8eaf645d48be/TP-7-41-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d55/5369273/adbef369c468/TP-7-41-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d55/5369273/e5fe1ba51081/TP-7-41-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d55/5369273/34eb5daaf894/TP-7-41-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d55/5369273/d29e3406f73d/TP-7-41-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d55/5369273/88938a2ec375/TP-7-41-g007.jpg

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