人骨髓间充质干细胞矿化过程中XV型胶原蛋白的免疫电子显微镜定位
Immunoelectron microscopic localization of Collagen type XV during human mesenchymal stem cells mineralization.
作者信息
Manferdini Cristina, Zini Nicoletta, Gabusi Elena, Paolella Francesca, Lambertini Elisabetta, Penolazzi Letizia, Piva Roberta, Lisignoli Gina
机构信息
a SC Laboratory of Immunorheumatology and Tissue Regeneration , Rizzoli Orthopaedic Institute , Bologna , Italy.
b CNR-National Research Council of Italy , IGM , Bologna , Italy.
出版信息
Connect Tissue Res. 2018 Dec;59(sup1):42-45. doi: 10.1080/03008207.2017.1408600.
UNLABELLED
Purpose/Aim of the study. Collagen type XV (ColXV) was identified, in our previews studies, as a novel component of bone extracellular matrix. The present study aims to investigate ColXV localization during mineralization of osteodifferentiated human mesenchymal stem cells (hMSCs).
MATERIAL AND METHODS
hMSCs cultured in osteogenic medium have been analyzed at day 14 and 28 for mineral matrix deposition by alizarin red S staining, ultrastructural analysis and ColXV localization by immunogold electron microscopy.
RESULTS
Our data show an intimate association between ColXV and fibrillar components in areas localized far from mineralized nodules.
CONCLUSIONS
We have demonstrated the efficacy of ultrastructural analysis, combined with immunocytochemistry, to establish a temporal and spatial localization of ColXV. This data, added to previous evidences, contribute to validate the negative effects of calcium deposits on ColXV expression.
未标注
研究目的。在我们之前的研究中，XV型胶原蛋白（ColXV）被鉴定为骨细胞外基质的一种新成分。本研究旨在调查成骨分化的人间充质干细胞（hMSCs）矿化过程中ColXV的定位。
材料与方法
通过茜素红S染色、超微结构分析以及免疫金电子显微镜对ColXV进行定位，在第14天和第28天对在成骨培养基中培养的hMSCs进行矿化基质沉积分析。
结果
我们的数据显示，在远离矿化结节的区域，ColXV与纤维状成分密切相关。
结论
我们已经证明超微结构分析与免疫细胞化学相结合，可用于确定ColXV的时空定位。这些数据，加上之前的证据，有助于验证钙沉积对ColXV表达的负面影响。
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