Manferdini Cristina, Zini Nicoletta, Gabusi Elena, Paolella Francesca, Lambertini Elisabetta, Penolazzi Letizia, Piva Roberta, Lisignoli Gina
a SC Laboratory of Immunorheumatology and Tissue Regeneration , Rizzoli Orthopaedic Institute , Bologna , Italy.
b CNR-National Research Council of Italy , IGM , Bologna , Italy.
Connect Tissue Res. 2018 Dec;59(sup1):42-45. doi: 10.1080/03008207.2017.1408600.
Purpose/Aim of the study. Collagen type XV (ColXV) was identified, in our previews studies, as a novel component of bone extracellular matrix. The present study aims to investigate ColXV localization during mineralization of osteodifferentiated human mesenchymal stem cells (hMSCs).
hMSCs cultured in osteogenic medium have been analyzed at day 14 and 28 for mineral matrix deposition by alizarin red S staining, ultrastructural analysis and ColXV localization by immunogold electron microscopy.
Our data show an intimate association between ColXV and fibrillar components in areas localized far from mineralized nodules.
We have demonstrated the efficacy of ultrastructural analysis, combined with immunocytochemistry, to establish a temporal and spatial localization of ColXV. This data, added to previous evidences, contribute to validate the negative effects of calcium deposits on ColXV expression.
研究目的。在我们之前的研究中，XV型胶原蛋白（ColXV）被鉴定为骨细胞外基质的一种新成分。本研究旨在调查成骨分化的人间充质干细胞（hMSCs）矿化过程中ColXV的定位。
通过茜素红S染色、超微结构分析以及免疫金电子显微镜对ColXV进行定位，在第14天和第28天对在成骨培养基中培养的hMSCs进行矿化基质沉积分析。
我们的数据显示，在远离矿化结节的区域，ColXV与纤维状成分密切相关。
我们已经证明超微结构分析与免疫细胞化学相结合，可用于确定ColXV的时空定位。这些数据，加上之前的证据，有助于验证钙沉积对ColXV表达的负面影响。
