Biomaterials, Biomechanics and Tissue Engineering Group (BBT), Department of Materials Science and Metallurgical Engineering, Universitat Politècnica de Catalunya (UPC), 08019, Barcelona, Spain; Barcelona Research Center in Multiscale Science and Engineering, UPC, 08019, Barcelona, Spain.
Biomaterials, Biomechanics and Tissue Engineering Group (BBT), Department of Materials Science and Metallurgical Engineering, Universitat Politècnica de Catalunya (UPC), 08019, Barcelona, Spain; Barcelona Research Center in Multiscale Science and Engineering, UPC, 08019, Barcelona, Spain; Institute for Bioengineering of Catalonia (IBEC), Barcelona Institute of Technology (BIST), 08028, Barcelona, Spain.
Colloids Surf B Biointerfaces. 2018 Sep 1;169:30-40. doi: 10.1016/j.colsurfb.2018.04.050. Epub 2018 Apr 27.
Strategies to inhibit initial bacterial adhesion are extremely important to prevent infection on biomaterial surfaces. However, the simultaneous attraction of desired eukaryotic cells remains a challenge for successful biomaterial-host tissue integration. Here we describe a method for the development of a trifunctional coating that repels contaminating bacteria, kills those that adhere, and promotes osteoblast adhesion. To this end, titanium surfaces were functionalized by electrodeposition of an antifouling polyethylene glycol (PEG) layer and subsequent binding of a peptidic platform with cell-adhesive and bactericidal properties. The physicochemical characterization of the samples via SEM, contact angle, FTIR and XPS analysis verified the successful binding of the PEG layer and the biomolecules, without altering the morphology and topography of the samples. PEG coatings inhibited protein adsorption and osteoblast-like (SaOS-2) attachment; however, the presence of cell adhesive domains rescued osteoblast adhesion, yielding higher values of cell attachment and spreading compared to controls (p < 0.05). Finally, the antibacterial potential of the coating was measured by live/dead assays and SEM using S. sanguinis as a model of early colonizer in oral biofilms. The presence of PEG layers significantly reduced bacterial attachment on the surfaces (p < 0.05). This antibacterial potential was further increased by the bactericidal peptide, yielding values of bacterial adhesion below 0.2% (p < 0.05). The balance between the risk of infection and the optimal osteointegration of a biomaterial is often described as "the race for the surface", in which contaminating bacteria and host tissue cells compete to colonize the implant. In the present work, we have developed a multifunctional coating for a titanium surface that promotes the attachment and spreading of osteoblasts, while very efficiently inhibits bacterial colonization, thus holding promise for application in bone replacing applications.
抑制初始细菌黏附的策略对于防止生物材料表面感染极其重要。然而，同时吸引所需的真核细胞仍然是成功实现生物材料-宿主组织整合的挑战。在这里，我们描述了一种开发三功能涂层的方法，该涂层排斥污染细菌，杀死黏附的细菌，并促进成骨细胞黏附。为此，通过电沉积抗污的聚乙二醇（PEG）层并随后结合具有细胞黏附和杀菌性能的肽平台，对钛表面进行功能化。通过 SEM、接触角、FTIR 和 XPS 分析对样品的物理化学特性进行了表征，证实了 PEG 层和生物分子的成功结合，而不会改变样品的形貌和形貌。PEG 涂层抑制蛋白质吸附和成骨细胞样（SaOS-2）附着；然而，细胞黏附结构域的存在挽救了成骨细胞的黏附，与对照相比，细胞附着和铺展的数值更高（p<0.05）。最后，通过使用 S. sanguinis 作为口腔生物膜中早期定植者的模型，通过活/死测定和 SEM 测量涂层的抗菌潜力。PEG 层的存在显著降低了表面上的细菌附着（p<0.05）。通过杀菌肽进一步增加了这种抗菌潜力，导致细菌黏附值低于 0.2%（p<0.05）。生物材料感染风险和最佳骨整合之间的平衡通常被描述为“表面的竞赛”，在这种竞赛中，污染细菌和宿主组织细胞竞争定植植入物。在本工作中，我们开发了一种用于钛表面的多功能涂层，该涂层促进成骨细胞的附着和铺展，同时非常有效地抑制细菌定殖，因此有望应用于骨替代应用。
