Ranjbari Abbas, Azarbayjani Mohammad Ali, Yusof Ashril, Halim Mokhtar Abdul, Akbarzadeh Samad, Ibrahim Mohamed Yousif, Tarverdizadeh Bahman, Farzadinia Parviz, Hajiaghaee Reza, Dehghan Firouzeh
Department of Physical Education, Sanandaj Farhangyan University, Sanandaj, Iran.
Exercise Physiology Department, Faculty of Physical Education, Islamic Azad University, Central Tehran Branch, Tehran, Iran.
BMC Complement Altern Med. 2016 Mar 15;16:101. doi: 10.1186/s12906-016-1064-6.
Urtica dioica (UD) has been identified as a traditional herbal medicine. This study aimed to investigate the effect of UD extract and swimming activity on diabetic parameters through in vivo and in vitro experiments.
Adult WKY male rats were randomly distributed in nine groups: intact control, diabetic control, diabetic + 625 mg/kg, 1.25 g/kg UD, diabetic + 100 mg/kg Metformin, diabetic + swimming, diabetic + swimming 625 mg/kg, 1.25 g/kg UD, and diabetic +100 mg/kg Metformin + swimming. The hearts of the animals were punctured, and blood samples were collected for biochemical analysis. The entire pancreas was exposed for histologic examination. The effect of UD on insulin secretion by RIN-5F cells in 6.25 or 12.5 mM glucose dose was examined. Glucose uptake by cultured L6 myotubes was determined.
The serum glucose concentration decreased, the insulin resistance and insulin sensitivity significantly increased in treated groups. These changes were more pronounced in the group that received UD extract and swimming training. Regeneration and less beta cell damage of Langerhans islets were observed in the treated groups. UD treatment increased insulin secretion in the RIN-5F cells and glucose uptake in the L6 myotubes cells.
Swimming exercises accompanied by consuming UD aqueous extracts effectively improved diabetic parameters, repaired pancreatic tissues in streptozotocin-induced diabetics in vivo, and increased glucose uptake or insulin in UD-treated cells in vitro.
荨麻已被确认为一种传统草药。本研究旨在通过体内和体外实验研究荨麻提取物及游泳活动对糖尿病参数的影响。
成年雄性WKY大鼠随机分为九组：完整对照组、糖尿病对照组、糖尿病 + 625毫克/千克、1.25克/千克荨麻组、糖尿病 + 100毫克/千克二甲双胍组、糖尿病 + 游泳组、糖尿病 + 游泳 + 625毫克/千克、1.25克/千克荨麻组，以及糖尿病 + 100毫克/千克二甲双胍 + 游泳组。穿刺动物心脏，采集血样进行生化分析。暴露整个胰腺进行组织学检查。检测荨麻对6.25或12.5毫摩尔/升葡萄糖剂量下RIN - 5F细胞胰岛素分泌的影响。测定培养的L6肌管的葡萄糖摄取。
治疗组血清葡萄糖浓度降低，胰岛素抵抗和胰岛素敏感性显著增加。这些变化在接受荨麻提取物和游泳训练的组中更为明显。治疗组观察到胰岛的再生和较少的β细胞损伤。荨麻治疗增加了RIN - 5F细胞中的胰岛素分泌和L6肌管细胞中的葡萄糖摄取。
饮用荨麻水提取物并进行游泳锻炼可有效改善糖尿病参数，修复链脲佐菌素诱导的糖尿病大鼠体内的胰腺组织，并增加体外荨麻处理细胞中的葡萄糖摄取或胰岛素分泌。
