Department of Chemical and Biomolecular Engineering , National University of Singapore , Singapore 117585.
Department of Surgery-Transplant and Mary & Dick Holland Regenerative Medicine Program , University of Nebraska Medical Center , Omaha , Nebraska 68198 , United States.
ACS Appl Mater Interfaces. 2018 Jun 6;10(22):18499-18506. doi: 10.1021/acsami.8b02914. Epub 2018 May 24.
Our previous work demonstrated the uniform particle pattern formation on the substrates using electrical field guided electrospray deposition. In this work, we reported for the first time the fabrication of gradient particle patterns on glass slides using an additional point, line, or bar electrode based on our previous electrospray deposition configuration. We also demonstrated that the polydimethylsiloxane (PDMS) coating could result in the formation of uniform particle patterns instead of gradient particle patterns on glass slides using the same experimental setup. Meanwhile, we investigated the effect of experimental configurations on the gradient particle pattern formation by computational simulation. The simulation results are in line with experimental observations. The formation of gradient particle patterns was ascribed to the gradient of electric field and the corresponding focusing effect. Cell patterns can be formed on the particle patterns deposited on PDMS-coated glass slides. The formed particle patterns hold great promise for high-throughput screening of biomaterial-cell interactions and sensing.
我们之前的工作展示了使用电场引导电喷雾沉积在基底上形成均匀的粒子图案。在这项工作中，我们首次报道了在玻璃载片上基于我们之前的电喷雾沉积装置通过附加的点、线或棒电极来制备梯度粒子图案。我们还证明了聚二甲基硅氧烷（PDMS）涂层可以在使用相同实验装置的情况下在玻璃载片上形成均匀的粒子图案，而不是梯度的粒子图案。同时，我们通过计算模拟研究了实验配置对梯度粒子图案形成的影响。模拟结果与实验观察结果一致。梯度粒子图案的形成归因于电场的梯度和相应的聚焦效应。可以在沉积在 PDMS 涂层的玻璃载片上的粒子图案上形成细胞图案。形成的粒子图案有望用于高通量筛选生物材料-细胞相互作用和传感。
