Yan Zishuo, Xie Jingwei
Department of Surgery-Transplant and Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE, 68198, USA.
Department of Mechanical and Materials Engineering, University of Nebraska Lincoln, Lincoln, NE, 68588, USA.
Macromol Biosci. 2025 Sep;25(9):e00151. doi: 10.1002/mabi.202500151. Epub 2025 May 14.
The selective deposition of electrospun nanofibers to form fiber patterns on the substrates is a challenge. This study reports a simple and robust approach for the fabrication of electrospun nanofiber microarrays on different substrates, including aluminum foil, paper, glass slide, and polydimethylsiloxane (PDMS), using a modified electrospinning setup. Formation of cell patterns on arrayed nanofibers, which are deposited on the PDMS substrate, is also demonstrated. The nanofiber microarrays could be used as a better substrate for cell culture compared to the current culture plate or dish, as electrospun nanofibers can mimic the architecture of the extracellular matrix. Nanofiber microarrays and cell microarrays developed in this study could be used for high-throughput sensing, drug/nanofiber formulation screening, and tissue patterning applications.
在基底上选择性沉积电纺纳米纤维以形成纤维图案是一项挑战。本研究报告了一种简单且稳健的方法，使用改良的电纺装置在不同基底上制备电纺纳米纤维微阵列，这些基底包括铝箔、纸张、载玻片和聚二甲基硅氧烷（PDMS）。还展示了在沉积于PDMS基底上的阵列纳米纤维上形成细胞图案。与当前的培养板或培养皿相比，纳米纤维微阵列可作为更好的细胞培养基底，因为电纺纳米纤维能够模拟细胞外基质的结构。本研究中开发的纳米纤维微阵列和细胞微阵列可用于高通量传感、药物/纳米纤维制剂筛选以及组织图案化应用。
