Korchak Sergey, Yang Shengjun, Mamone Salvatore, Glöggler Stefan
NMR Signal Enhancement Group Max-Planck-Institute for Biophysical Chemistry Am Faßberg 11 37077 Göttingen Germany.
Center for Biostructural Imaging of Neurodegeneration of UMG Von-Siebold-Str. 3A 37075 Göttingen Germany.
ChemistryOpen. 2018 May 8;7(5):344-348. doi: 10.1002/open.201800024. eCollection 2018 May.
Diseases such as Alzheimer's and cancer have been linked to metabolic dysfunctions, and further understanding of metabolic pathways raises hope to develop cures for such diseases. To broaden the knowledge of metabolisms in vitro and in vivo, methods are desirable for direct probing of metabolic function. Here, we are introducing a pulsed nuclear magnetic resonance (NMR) approach to generate hyperpolarized metabolites within seconds, which act as metabolism probes. Hyperpolarization represents a magnetic resonance technique to enhance signals by over 10 000-fold. We accomplished an efficient metabolite hyperpolarization by developing an isotopic labeling strategy for generating precursors containing a favorable nuclear spin system to add -hydrogen and convert its two-spin longitudinal order into enhanced metabolite signals. The transfer is performed by an invented NMR experiment and 20 000-fold signal enhancements are achieved. Our technique provides a fast way of generating hyperpolarized metabolites by using -hydrogen directly in a high magnetic field without the need for field cycling.
阿尔茨海默病和癌症等疾病已被证实与代谢功能障碍有关，对代谢途径的进一步了解为开发此类疾病的治疗方法带来了希望。为了拓宽对体外和体内代谢的认识，需要直接探测代谢功能的方法。在此，我们介绍一种脉冲核磁共振（NMR）方法，可在数秒内生成超极化代谢物，这些代谢物可作为代谢探针。超极化是一种磁共振技术，可将信号增强超过10000倍。我们通过开发一种同位素标记策略实现了高效的代谢物超极化，该策略用于生成含有有利核自旋系统的前体，以添加氢并将其二自旋纵向顺序转化为增强的代谢物信号。这种转移通过一项发明的核磁共振实验来完成，实现了20000倍的信号增强。我们的技术提供了一种在高磁场中直接使用氢快速生成超极化代谢物的方法，无需场循环。
