Department of Chemistry, Duke University , Durham, North Carolina 27708, United States.
Institute for Technical and Macromolecular Chemistry, RWTH Aachen University , Worringerweg 2, 52072 Aachen, Germany.
J Am Chem Soc. 2017 Jun 14;139(23):7761-7767. doi: 10.1021/jacs.7b00569. Epub 2017 May 30.
Signal amplification by reversible exchange (SABRE) is an inexpensive, fast, and even continuous hyperpolarization technique that uses para-hydrogen as hyperpolarization source. However, current SABRE faces a number of stumbling blocks for translation to biochemical and clinical settings. Difficulties include inefficient polarization in water, relatively short-lived H-polarization, and relatively limited substrate scope. Here we use a water-soluble polarization transfer catalyst to hyperpolarize nitrogen-15 in a variety of molecules with SABRE-SHEATH (SABRE in shield enables alignment transfer to heteronuclei). This strategy works in pure HO or DO solutions, on substrates that could not be hyperpolarized in traditional H-SABRE experiments, and we record N T relaxation times of up to 2 min.
信号放大可逆交换（SABRE）是一种廉价、快速甚至连续的极化技术，它使用仲氢作为极化源。然而，目前的 SABRE 在向生化和临床环境转化方面面临着许多障碍。困难包括水相中的极化效率低、H 极化的寿命相对较短以及底物范围相对有限。在这里，我们使用一种水溶性极化转移催化剂，通过 SABRE-SHEATH（屏蔽中的 SABRE 可实现向杂核的对准转移）来极化各种分子中的氮-15。该策略在纯 HO 或 DO 溶液中、在传统 H-SABRE 实验中无法极化的底物上均有效，并且我们记录到的 N T 弛豫时间长达 2 分钟。
