Department of Hand surgery, Yantaishan Hospital, Yantai, China.
Eur Rev Med Pharmacol Sci. 2018 Apr;22(8):2439-2446. doi: 10.26355/eurrev_201804_14837.
This study aimed at investigating the role of Neurotrophin-3 (NT-3) in the bone fracture healing of rats and to provide a reference for clinical treatment.
40 Sprague-Dawley (SD) rats were randomly divided into control group and NT-3 group. The tibia fracture model was made in NT-3 group, and the tibia bone mineral density (BMD) was measured before and after the surgery. The biomechanics indexes were inspected after the surgery, including elasticity modulus, max load, and bending rigidity. The levels of bone morphogenetic protein (BMP)-2 and transforming growth factor (TGF)-β1 in serum were examined by enzyme-linked immunosorbent assay (ELISA). Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the levels of hypoxia-inducible factor (HIF)-1α and vascular endothelial growth factor (VEGF) mRNA expression in callus tissue. The pathological profile of tibia fracture healing was characterized after the surgery.
The levels of BMD in NT-3 group were significantly higher than that in control group after the surgery (p < 0.05). The levels of elasticity modulus, maximum load, stiffness of shinbone, BMP-2 and TGF-β1 were significantly higher in NT-3 group than those in control group after the surgery (p < 0.05). Compared with the control group, the expression of HIF-1α mRNA was significantly lower and the expression of VEGF mRNA was significantly higher in NT-3 group after the surgery (p < 0.05). Histological study showed that the periosteal reaction, capillary proliferation, cartilage cells production and ossification were happened after treating NT-3 for tibia fracture model rats.
NT-3 can significantly improve fracture healing in rats.
本研究旨在探讨神经营养因子-3（NT-3）在大鼠骨骨折愈合中的作用，为临床治疗提供参考。
40 只 Sprague-Dawley（SD）大鼠随机分为对照组和 NT-3 组。在 NT-3 组制作胫骨骨折模型，手术前后测量胫骨骨密度（BMD）。手术后检查生物力学指标，包括弹性模量、最大载荷和弯曲刚度。采用酶联免疫吸附试验（ELISA）检测血清中骨形态发生蛋白（BMP）-2 和转化生长因子（TGF）-β1 的水平。采用逆转录-聚合酶链反应（RT-PCR）检测骨痂组织中缺氧诱导因子（HIF）-1α和血管内皮生长因子（VEGF）mRNA 表达水平。手术后对胫骨骨折愈合的病理特征进行描述。
手术后 NT-3 组的 BMD 水平明显高于对照组（p < 0.05）。手术后 NT-3 组的弹性模量、最大载荷、胫骨刚度、BMP-2 和 TGF-β1 水平明显高于对照组（p < 0.05）。与对照组相比，手术后 NT-3 组 HIF-1α mRNA 的表达明显降低，VEGF mRNA 的表达明显升高（p < 0.05）。组织学研究表明，NT-3 治疗大鼠胫骨骨折模型后，骨膜反应、毛细血管增殖、软骨细胞生成和骨化发生。
NT-3 能显著改善大鼠骨折愈合。
