Huo Yongjiu, Liu Zhengxu, Xuan Han, Lu Chunbin, Yu Lihuai, Bao Wenbin, Zhao Guoqi
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Animal Disease Control and Prevention Centre of Jiangyin City, Jiangyin 214400, China.
Anim Nutr. 2016 Jun;2(2):111-118. doi: 10.1016/j.aninu.2016.02.006. Epub 2016 Mar 14.
The aim of this study was to explore the effects of bamboo vinegar powder on growth performance, diarrhea situation and mRNA expression levels of cytokines i.e., interleukin-10 (), interleukin-22 (), and interleukin-25 () in immune organs of weaned piglets, and to accumulate theoretical data for the application of bamboo vinegar powder in weaned piglet production. Forty-five crossbred (Duroc × Landrace × Yorkshire, all male) weaned piglets with similar body weight (6.74 ± 0.17 kg) at 31 days of age were randomly assigned to 5 treatments with 3 replicates per treatment and 3 piglets in each replicate. The five treatments were as follows: CON (a basal diet), ANT (the basal diet + 0.12% antibiotics), BV1 (the basal diet + 0.1% bamboo vinegar powder), BV5 (the basal diet + 0.5% bamboo vinegar powder), BV10 (the basal diet + 1.0% bamboo vinegar powder). This experiment lasted 35 days. The growth performance and diarrhea situation were recorded. The relative mRNA expression levels of , and in liver, spleen, duodenum and mesenteric lymph nodes were detected by real-time PCR. Feed: gain of BV5 was significantly lower than that of CON ( < 0.05). In comparison with CON, diarrhea rate and diarrhea index of BV1 and BV5 all tended to decrease ( < 0.1). Compared with CON, mRNA expression level of in liver of ANT tended to be lower ( < 0.1) and these of BV1, BV5 and BV10 were significantly reduced ( < 0.05). The mRNA expression levels of in duodenum of ANT, BV1, BV5 and BV10 were all lower than those of CON, of which BV10 had significantly decreased mRNA expression in duodenum ( < 0.05). The mRNA expression levels of in duodenum of ANT, BV1, BV5 and BV10 all tended to be inhibited compared with CON ( < 0.1). With the increase of bamboo vinegar powder dosage, mRNA expression levels of in spleen and mesenteric lymph nodes of BV1, BV5 and BV10 tended to be up-regulated. Overall, bamboo vinegar powder could improve growth performance, and regulate mRNA expression levels of , and in immune organs of weaned piglets. The dosage at 0.5% showed optimum effects.
本研究旨在探讨竹醋粉对断奶仔猪生长性能、腹泻情况以及免疫器官中细胞因子白细胞介素 -10（IL-10）、白细胞介素 -22（IL-22）和白细胞介素 -25（IL-25）mRNA表达水平的影响，为竹醋粉在断奶仔猪生产中的应用积累理论数据。选取45头31日龄体重相近（6.74±0.17 kg）的杜洛克×长白×约克夏杂交公断奶仔猪，随机分为5组，每组3个重复，每个重复3头仔猪。5组处理分别为：CON（基础日粮）、ANT（基础日粮 + 0.12%抗生素）、BV1（基础日粮 + 0.1%竹醋粉）、BV5（基础日粮 + 0.5%竹醋粉）、BV10（基础日粮 + 1.0%竹醋粉）。本试验持续35天。记录生长性能和腹泻情况。通过实时荧光定量PCR检测肝脏、脾脏、十二指肠和肠系膜淋巴结中IL-10、IL-22和IL-25的相对mRNA表达水平。BV5组的料重比显著低于CON组（P<0.05）。与CON组相比，BV1组和BV5组的腹泻率和腹泻指数均有下降趋势（P<0.1）。与CON组相比，ANT组肝脏中IL-10的mRNA表达水平有下降趋势（P<0.1），BV1组、BV5组和BV10组显著降低（P<0.05）。ANT组、BV1组、BV5组和BV10组十二指肠中IL-10的mRNA表达水平均低于CON组，其中BV10组十二指肠中IL-10的mRNA表达显著降低（P<0.05）。与CON组相比，ANT组、BV1组、BV5组和BV10组十二指肠中IL-22的mRNA表达水平均有被抑制的趋势（P<0.1）。随着竹醋粉添加量的增加，BV1组、BV5组和BV10组脾脏和肠系膜淋巴结中IL-25的mRNA表达水平有上调趋势。总体而言，竹醋粉可改善断奶仔猪的生长性能，并调节其免疫器官中IL-10、IL-22和IL-25的mRNA表达水平。0.5%的添加量效果最佳。
