沸石和过磷酸钙作为添加剂对工厂化鸡粪堆肥过程中抗生素抗性基因和细菌群落的影响。
Influence of zeolite and superphosphate as additives on antibiotic resistance genes and bacterial communities during factory-scale chicken manure composting.
机构信息
State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, Jiangsu 210008, China; College of Environment and Ecology, Jiangsu Open University, Nanjing, Jiangsu 210017, China.
State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, Jiangsu 210008, China.
出版信息
Bioresour Technol. 2018 Sep;263:393-401. doi: 10.1016/j.biortech.2018.04.107. Epub 2018 Apr 30.
Factory-scale chicken manure composting added with zeolite (F), superphosphate (G), or zeolite and ferrous sulfate (FL) simultaneously, were evaluate for their effects on the behaviors of antibiotic resistance genes (ARGs) and bacterial communities. After composting, ARGs in manure decreased by 67.3% in the control, whereas the reductions were 86.5%, 68.6% and 72.2% in F, G and FL, respectively. ARGs encoding ribosomal protection proteins (tetO, tetB(P), and tetM) were reduced to a greater extent than tetG, tetL, sul1 and sul2. Bacteria pathogens were also effectively removed by composting. Network analysis showed that Firmicutes were the important potential host bacteria for ARGs. The bacterial communities and environmental factors, as well as the intI gene, contributed significantly to the variation of ARGs. The ARGs and integrons were reduced more when zeolite was added than when superphosphate was added; thus, it may be useful for reducing the risks of ARGs in chicken manure.
工厂规模的鸡粪堆肥添加沸石（F）、过磷酸钙（G）或沸石和硫酸亚铁（FL），评估它们对抗生素抗性基因（ARGs）和细菌群落行为的影响。堆肥后，对照处理中粪肥中的 ARGs 减少了 67.3%，而 F、G 和 FL 处理中的减少率分别为 86.5%、68.6%和 72.2%。编码核糖体保护蛋白（tetO、tetB(P) 和 tetM）的 ARGs 比 tetG、tetL、sul1 和 sul2 减少的幅度更大。细菌病原体也通过堆肥有效地去除。网络分析表明，厚壁菌门是 ARGs 的重要潜在宿主细菌。细菌群落和环境因素以及 intI 基因对 ARGs 的变异有显著贡献。添加沸石比添加过磷酸钙时，ARGs 和整合子减少更多；因此，它可能有助于降低鸡粪中 ARGs 的风险。
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