Estación Biológica de Doñana (EBD-CSIC), Calle Américo Vespucio 26, E-41092, Seville, Spain.
CIBER de Epidemiología y Salud Pública (CIBERESP), Seville, Spain.
Parasit Vectors. 2018 May 18;11(1):297. doi: 10.1186/s13071-018-2879-4.
Aedes vittatus is currently found in Africa, Asia and Europe, where it acts as a vector of pathogens causing animal and human diseases (e.g. chikungunya, Zika and dengue). Like other Aedes species, Ae. vittatus is able to breed in artificial containers. The ECDC has recently highlighted the need for molecular tools (i.e. barcoding characterization) that enable Aedes species to be identified in entomological surveys.
We sampled mosquito larvae and adults in southern Spain and used a molecular approach to amplify and sequence a fragment of the cytochrome c oxidase subunit 1 gene (barcoding region) of the mosquitoes. The blast comparison of the mosquito sequences isolated from Spain with those deposited in public databases provided a ≥ 99% similarity with sequences for two Aedes mosquitoes, Ae. vittatus and Ae. cogilli, while similarities with other Aedes species were ≤ 94%. Aedes cogilli is only present in India and there are no records of this species from Europe.
Due to the low genetic differences between Ae. vittatus and Ae. cogilli, the barcoding region should not be used as the only method for identifying Ae. vittatus, especially in areas where both of these Aedes species are present. This type of analysis should thus be combined with morphological identification using available keys and/or the characterization of other molecular markers. In addition, further entomological surveys should be conducted in order to identify the fine-scale distribution of this mosquito species in Europe.
刺扰伊蚊目前分布于非洲、亚洲和欧洲，是携带动物和人类疾病病原体（如基孔肯雅热、寨卡和登革热）的媒介。与其他伊蚊属物种一样，刺扰伊蚊能够在人工容器中繁殖。欧洲疾病预防控制中心最近强调需要使用分子工具（即条形码特征描述）来识别昆虫学调查中的伊蚊属物种。
我们在西班牙南部采集了蚊子幼虫和成虫，并使用分子方法扩增和测序了蚊子细胞色素 c 氧化酶亚基 1 基因（条形码区域）的一个片段。从西班牙分离出的蚊子序列与公共数据库中保存的序列进行blast 比较，与两种伊蚊，即刺扰伊蚊和库蚊的序列相似度≥99%，而与其他伊蚊属物种的相似度≤94%。库蚊仅存在于印度，欧洲没有该物种的记录。
由于刺扰伊蚊和库蚊之间的遗传差异较低，因此条形码区域不应作为识别刺扰伊蚊的唯一方法，特别是在同时存在这两种伊蚊的地区。这种类型的分析应与使用现有鉴定钥匙进行的形态学鉴定和/或其他分子标记特征描述相结合。此外，应进一步开展昆虫学调查，以确定该蚊子在欧洲的细尺度分布。
