Key Laboratory of Aquatic Products Processing and Utilization of Liaoning Province, National R & D Branch Center for Seaweed Processing, College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, PR China.
Carbohydr Polym. 2018 Aug 1;193:298-306. doi: 10.1016/j.carbpol.2018.03.077. Epub 2018 Mar 23.
For illustrating the relation of different structural characteristics correlated with the hepatoprotective effect, studies of fucoidan from algae Kjellmaniella crassifolia, a brown alga distributed in north Japan, were carried out. The fucoidan fractions of K. crassifolia were extracted, separated, and purified using a combinatorial procedure consisting of enzymolysis, ethanol precipitation, DEAE and size-exclusion chromatographies. The fundamental characteristics of the four enriched fucoidan fractions (KF1-KF4), including their sulphate content and monosaccharide composition, were investigated. The fucose was the main composition of monosaccharide for KF1-KF4, that of KF4 was up to 91.4%. The Glu-UA was the special composition of monosaccharide. FTIR and NMR spectroscopy were employed to elucidate the structural features of all fractions which further illustrated that fucose was the main monosaccharide. It was found all the four fractions showed antioxidative activity against hydroxyl radical and had the bioactive effects on CCl-induced liver injury.
为了阐明与肝保护作用相关的不同结构特征之间的关系，对分布在日本北部的褐藻 Kjellmaniella crassifolia 中的岩藻聚糖进行了研究。使用包括酶解、乙醇沉淀、DEAE 和分子筛层析在内的组合程序从 K. crassifolia 中提取、分离和纯化岩藻聚糖级分。研究了四个富岩藻聚糖级分（KF1-KF4）的基本特性，包括硫酸根含量和单糖组成。KF1-KF4 的主要单糖组分为岩藻糖，KF4 中的岩藻糖含量高达 91.4%。单糖的特殊组分为 Glu-UA。采用傅里叶变换红外光谱（FTIR）和核磁共振波谱（NMR）光谱阐明了所有级分的结构特征，进一步表明岩藻糖是主要的单糖。结果发现，所有四个级分均显示出对羟基自由基的抗氧化活性，并且对 CCl 诱导的肝损伤具有生物活性作用。
