Ajisaka Katsumi, Yokoyama Tatsuya, Matsu Keita
1 Department of Applied Life Science, Niigata University of Pharmacy and Applied Life Sciences.
J Appl Glycosci (1999). 2016 Mar 17;63(2):31-37. doi: 10.5458/jag.jag.JAG-2015_024. eCollection 2016.
Five kinds of fucoidans from the brown seaweeds , , (), , and , were isolated according to a previously reported procedure with slight modification. The scavenging activities of DPPH radical, superoxide radical, and hydroxyl radical, as well as the ORAC value were measured for the isolated fucoidans. Fucoidans from , , and showed higher antioxidant activity than that from and , except for the hydroxyl radical scavenging activity. The relationship between the antioxidant activity and the structure was examined for each fucoidan. Fucoidans with high amount of sulfate groups did not necessarily result in increased antioxidant activity, although the sulfate group itself was essential for the antioxidant activity. Furthermore, the fucoidan linked to a side chain monosaccharide, such as GlcA, demonstrated similar antioxidant activity. The antioxidant activity of the fucoidans was possibly due to a combination of the factors involved, such as the amount of sulfate groups, the position of the sulfate groups, the kind of side chain sugar, the linkage of a side chain sugar, and the molecular weight.
按照之前报道的方法并稍作修改，从褐藻、、（）、和中分离出了五种岩藻聚糖。对分离出的岩藻聚糖测定了其对二苯基苦味酰基自由基（DPPH自由基）、超氧阴离子自由基和羟基自由基的清除活性以及氧自由基吸收能力（ORAC）值。除了羟基自由基清除活性外，来自、和的岩藻聚糖比来自和的岩藻聚糖表现出更高的抗氧化活性。对每种岩藻聚糖的抗氧化活性与其结构之间的关系进行了研究。尽管硫酸根本身对于抗氧化活性至关重要，但具有大量硫酸根的岩藻聚糖并不一定会导致抗氧化活性增加。此外，与侧链单糖（如葡萄糖醛酸）相连的岩藻聚糖表现出相似的抗氧化活性。岩藻聚糖的抗氧化活性可能是由多种因素共同作用导致的，如硫酸根的数量、硫酸根的位置、侧链糖的种类、侧链糖的连接方式以及分子量。
