IBSB, Department of Bioengineering, Marmara University, Istanbul, Turkey; Department of Bioengineering, Gebze Technical University, Kocaeli, Turkey.
Department of Medical Biology, Acıbadem University School of Medicine, Istanbul, Turkey.
Carbohydr Polym. 2018 Aug 1;193:316-325. doi: 10.1016/j.carbpol.2018.03.075. Epub 2018 Mar 23.
This represents the first systematic study where levan polysaccharide was used to fabricate fibrous matrices by co-axial and single-needle electrospinning techniques. For this, hydrolyzed (hHL) and sulfated hydrolyzed (ShHL) Halomonas levan were chemically synthesized and used together with polycaprolactone (PCL) and polyethyleneoxide (PEO) for the spinning process. In co-axially spun matrices, ultimate tensile strength (UTS) were found to increase with increasing ShHL concentration and elongation at break of PCL + ShHL matrices increased up to ten-fold when compared to PCL matrices. Similarly, in single-needle spun matrices, higher elongation at break values were obtained by blending HL and ShHL with PEO pointing to the effective energy absorbing features. Dense and fine fibers were characterized by FTIR and SEM. Cell viability and fluorescence imaging of L929 fibroblasts and HUVECs as well as heparin mimetic activity of the matrices pointed to their high potential to be used in decreasing neointimal proliferation and thrombogenicity of grafts and prosthesis.
这是首次采用Levan 多糖通过同轴和单针静电纺丝技术制备纤维基质的系统研究。为此，将水解（hHL）和硫酸化水解（ShHL）盐单胞菌 Levan 进行化学合成，并与聚己内酯（PCL）和聚氧化乙烯（PEO）一起用于纺丝过程。在同轴纺丝基质中，发现极限拉伸强度（UTS）随 ShHL 浓度的增加而增加，与 PCL 基质相比，PCL+ShHL 基质的断裂伸长率增加了十倍。同样，在单针纺丝基质中，通过将 HL 和 ShHL 与 PEO 混合，可以获得更高的断裂伸长率值，表明其具有有效的能量吸收特性。FTIR 和 SEM 对致密和细纤维进行了表征。L929 成纤维细胞和 HUVECs 的细胞活力和荧光成像以及基质的肝素模拟活性表明，它们具有降低移植物和假体新生内膜增殖和血栓形成的巨大潜力。
