Rong Hongtao, Fan Yueshan, Yang Mengchen, Zhang Baoliang, Sun Dongdong, Zhao Zilong, Wang Dong, Fan Weidong, Wang Jianhao, Gu Gang, Li Fanjian, Liu Xilei, Rao Chenxu, Chen Huijiao, Wang Yi, Tian Ye, Zhang Jianning
Tianjin Medical University General Hospital, No. 154, Anshan Road, Heping Distict, Tianjin 300052, China.
Tianjin Medical University General Hospital, No. 154, Anshan Road, Heping Distict, Tianjin 300052, China; Tianjin Medical University, No. 22, Qixiangtai Road, Heping Distict, Tianjin 300070, China.
Brain Res. 2018 Sep 1;1694:104-110. doi: 10.1016/j.brainres.2018.05.015. Epub 2018 May 16.
Microparticles are cell fragments derived from damaged cells that are able to present an antigen from the parent cells to other cells to activate intracellular signaling pathways. Microparticles are closely related to the inflammatory response. Brain-derived microparticles (BDMPs) play an important role in brain injury. However, the inflammatory effect of BDMPs on microglia/macrophages remains unclear. The BDMPs were consumed by microglia/macrophages in vivo and in vitro. The BDMPs activated microglia/macrophages and changed their morphology in vitro. The BDMPs dysregulate the production of pro-inflammatory factors, suggesting that the effect of the BDMPs on microglia/macrophages is pro-inflammatory. In this study, we used flow cytometry, hopping probe ion conductance microscopy, immunofluorescence and other techniques to study the effect of brain-derived microparticle activation on microglia/macrophages that leads to neuroinflammation. BDMPs might be possible targets for the treatment of traumatic brain injury (TBI) changes after secondary nerve inflammation.
微粒是源自受损细胞的细胞碎片，能够将来自亲代细胞的抗原呈递给其他细胞，以激活细胞内信号通路。微粒与炎症反应密切相关。脑源性微粒（BDMPs）在脑损伤中起重要作用。然而，BDMPs对小胶质细胞/巨噬细胞的炎症作用仍不清楚。BDMPs在体内和体外均被小胶质细胞/巨噬细胞摄取。BDMPs在体外激活小胶质细胞/巨噬细胞并改变其形态。BDMPs使促炎因子的产生失调，这表明BDMPs对小胶质细胞/巨噬细胞的作用是促炎的。在本研究中，我们使用流式细胞术、跳跃探针离子电导显微镜、免疫荧光等技术，研究脑源性微粒激活对导致神经炎症的小胶质细胞/巨噬细胞的影响。BDMPs可能是治疗继发性神经炎症后创伤性脑损伤（TBI）变化的潜在靶点。
