Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Wenhua Xi Road No. 44-1, Jinan, 250012, Shandong, PR China.
Department of Orthodontics, School of Stomatology, Shandong University, Jinan, Shandong, PR China.
J Mater Sci Mater Med. 2019 Dec 24;31(1):11. doi: 10.1007/s10856-019-6349-0.
Polyether-ether-ketone (PEEK) is becoming a popular component of clinical spinal and orthopedic applications, but its practical use suffers from several limitations. In this study, irregular nano-porous monolayer with differently functional groups was formed on the surface of PEEK through sulfonation and nitrification. The surface characteristics were detected by field-emission scanning electron microscopy, atomic force microscopy, energy-dispersive X-ray spectrometry, water contact angle measurements and Fourier transform infrared spectroscopy. In vitro cellular behaviors were evaluated by cell adhesion, morphological changes, proliferation, alkalinity, phosphatase activity, real-time RT-PCR and western blot analyses. In vivo osseointegration was examined through micro-CT and histological assessments. Our results reveal that the irregular nano-porous of PEEK affect the biological properties. High-temperature hydrothermal NP treatment induced early osteogenic differentiation and early osteogenesis. Modification by sulfonation and nitrification can broaden the use of PEEK in orthopedic and dental applications. This study provides a theoretical basis for the wider clinical application of PEEK. a To obtain a uniform porous structure, PEEK samples were treated by concentrated sulfuric acid and fuming nitric acid (82-80%) with magnetic stirring sequentially. b Effects of nanopores on biological behavior of bMSCS.
聚醚醚酮（PEEK）正成为临床脊柱和骨科应用中一种很受欢迎的组件，但其实用性受到多种限制。在这项研究中，通过磺化和硝化在 PEEK 表面形成具有不同官能团的不规则纳米多孔单层。通过场发射扫描电子显微镜、原子力显微镜、能量色散 X 射线能谱、水接触角测量和傅里叶变换红外光谱检测表面特性。通过细胞黏附、形态变化、增殖、碱性、碱性磷酸酶活性、实时 RT-PCR 和 Western blot 分析评估体外细胞行为。通过 micro-CT 和组织学评估检查体内骨整合。我们的结果表明，PEEK 的不规则纳米多孔结构会影响其生物特性。高温水热处理 NP 诱导早期成骨分化和早期成骨。磺化和硝化改性可以拓宽 PEEK 在骨科和牙科应用中的用途。本研究为 PEEK 的更广泛临床应用提供了理论依据。a 为了获得均匀的多孔结构，PEEK 样品用硫酸（浓度 82-80%）和发烟硝酸（浓度 82-80%）在有磁场搅拌的条件下依次处理。b 纳米孔对骨髓间充质干细胞（bMSCS）生物学行为的影响。
