Gajigan Andrian P, Yñiguez Aletta T, Villanoy Cesar L, San Diego-McGlone Maria Lourdes, Jacinto Gil S, Conaco Cecilia
Marine Science Institute, University of the Philippines Diliman, Quezon City, Philippines.
Current affiliation: Department of Oceanography, University of Hawaii at Manoa, USA.
PeerJ. 2018 May 16;6:e4781. doi: 10.7717/peerj.4781. eCollection 2018.
Microbes are central to the structuring and functioning of marine ecosystems. Given the remarkable diversity of the ocean microbiome, uncovering marine microbial taxa remains a fundamental challenge in microbial ecology. However, there has been little effort, thus far, to describe the diversity of marine microorganisms in the region of high marine biodiversity around the Philippines. Here, we present data on the taxonomic diversity of bacteria and archaea in Benham Rise, Philippines, Western Pacific Ocean, using 16S V4 rRNA gene sequencing. The major bacterial and archaeal phyla identified in the Benham Rise are Proteobacteria, Cyanobacteria, Actinobacteria, Bacteroidetes, Marinimicrobia, Thaumarchaeota and, Euryarchaeota. The upper mesopelagic layer exhibited greater microbial diversity and richness compared to surface waters. Vertical zonation of the microbial community is evident and may be attributed to physical stratification of the water column acting as a dispersal barrier. Canonical Correspondence Analysis (CCA) recapitulated previously known associations of taxa and physicochemical parameters in the environment, such as the association of oligotrophic clades with low nutrient surface water and deep water clades that have the capacity to oxidize ammonia or nitrite at the upper mesopelagic layer. These findings provide foundational information on the diversity of marine microbes in Philippine waters. Further studies are warranted to gain a more comprehensive picture of microbial diversity within the region.
微生物对于海洋生态系统的结构和功能至关重要。鉴于海洋微生物群落的显著多样性，揭示海洋微生物分类群仍然是微生物生态学中的一项基本挑战。然而，迄今为止，在描述菲律宾周围海洋生物多样性高的区域内海洋微生物的多样性方面几乎没有做出什么努力。在这里，我们利用16S V4 rRNA基因测序技术，展示了西太平洋菲律宾本哈姆海隆细菌和古菌的分类多样性数据。在本哈姆海隆鉴定出的主要细菌和古菌门包括变形菌门、蓝细菌门、放线菌门、拟杆菌门、海洋微生物门、奇古菌门和广古菌门。与表层水相比，中上层表现出更高的微生物多样性和丰富度。微生物群落的垂直分区很明显，这可能归因于水柱的物理分层起到了扩散屏障的作用。典范对应分析（CCA）概括了先前已知的分类群与环境中理化参数的关联，例如贫营养类群与低营养表层水的关联，以及在中上层具有氧化氨或亚硝酸盐能力的深水类群。这些发现为菲律宾海域海洋微生物的多样性提供了基础信息。有必要进行进一步的研究，以更全面地了解该区域内的微生物多样性。
