Dept. of Food Science, Univ. of Massachusetts Amherst, 102 Holdsworth Way, Amherst, MA 01003, U.S.A.
St. David's School, 3400 White Oak Road, Raleigh, NC 27609, U.S.A.
J Food Sci. 2018 Jun;83(6):1631-1638. doi: 10.1111/1750-3841.14179. Epub 2018 May 22.
A simple, rapid, and specific colorimetric method for gentamicin detection using cysteamine-modified gold nanoparticles (cys-AuNPs) has been developed. The maximum residue limits of gentamicin allowed in foods are typically below 100 nM, so an effective detection method for low concentrations of the drug is required. The aggregation of gold nanoparticles (AuNPs) was used as the basis for this method, and adding cysteamine to the AuNPs helped to enhance their aggregative abilities. The cys-AuNPs are capable of detecting gentamicin concentrations as low as 12.45 nM in water, which could be quantified using UV-vis spectroscopy. Samples extracted from skim milk with a simple pretreatment showed that gentamicin concentrations down to at least 100 nM could be observed using the cys-AuNPs. This study demonstrates the ability of the cys-AuNPs to rapidly detect and quantify gentamicin in both simple and complex matrices.
This study demonstrates that cysteamine-modified gold nanoparticles could be used as a rapid and efficient tool for gentamicin detection. This technique is cheaper, simpler, and more effective than many other methods that are currently used for detecting the antibiotic in industrial and commercial applications. It has a great potential to be practically applied as a rapid screening method for gentamicin and gentamicin-like compounds in food and environmental samples.
本研究开发了一种使用半胱氨酸修饰的金纳米粒子（cys-AuNPs）检测庆大霉素的简单、快速且特异性的比色法。食品中允许的庆大霉素最大残留限量通常低于 100 nM，因此需要一种有效的低浓度药物检测方法。该方法基于金纳米粒子（AuNPs）的聚集，而向 AuNPs 中添加半胱氨酸有助于增强其聚集能力。cys-AuNPs 能够检测水中低至 12.45 nM 的庆大霉素浓度，这可以通过紫外-可见光谱进行定量。使用简单的预处理从脱脂乳中提取的样品表明，使用 cys-AuNPs 可以观察到至少 100 nM 的庆大霉素浓度。本研究证明了 cys-AuNPs 能够快速检测和定量简单和复杂基质中的庆大霉素。
本研究表明，半胱氨酸修饰的金纳米粒子可用作庆大霉素快速检测和定量的有效工具。与目前在工业和商业应用中用于检测抗生素的许多其他方法相比，该技术更便宜、更简单、更有效。它具有很大的实际应用潜力，可作为食品和环境样品中庆大霉素和类似庆大霉素化合物的快速筛选方法。
