Department of Pharmacy of Tongji Hospital, Tongji Medical School, Huazhong Science and Technology University, Wuhan, 430030, China.
Department of Paediatrics of Tongji Hospital, Tongji Medical School, Huazhong Science and Technology University, Wuhan, 430030, China.
Lipids Health Dis. 2018 May 23;17(1):121. doi: 10.1186/s12944-018-0774-9.
Bile acids (BAs) are important regulatory factors of life activities, which are involved in the regulation of glucose, lipid and energy metabolisms, and closely associated with intestinal hormones, microbiotas and energy balance. BAs abnormalities easily lead to inflammation and metabolic diseases, in turn, the progress of diseases could influence characteristics of BAs. Therefore, accurate detection of BAs contents is of great significance to disease prevention, diagnosis and treatment. At present, the most widely used enzymatic method in clinical practice is applicable to the detection of total bile acid (TBA). In laboratory research, different types of BAs can be accurately separated and quantified by liquid chromatography-mass spectrometry (LC-MS). The metabolic profiling of BAs based on detection technologies can completely and accurately monitor their types and contents, playing a crucial role in disease prevention, diagnosis and treatment. We herein reviewed the main detection technologies of BAs and the application of metabolic profiling in related diseases in recent years.
胆汁酸（BAs）是生命活动的重要调节因子，参与调节糖、脂和能量代谢，与肠道激素、微生物群和能量平衡密切相关。BAs 异常容易导致炎症和代谢性疾病，反过来，疾病的进展也会影响 BAs 的特征。因此，准确检测 BAs 的含量对疾病的预防、诊断和治疗具有重要意义。目前，临床实践中最广泛使用的酶法适用于总胆汁酸（TBA）的检测。在实验室研究中，液相色谱-质谱联用（LC-MS）等技术可以准确分离和定量不同类型的 BAs。基于检测技术的 BAs 代谢组学可以完全准确地监测其类型和含量，在疾病的预防、诊断和治疗中发挥着至关重要的作用。本文综述了近年来 BAs 的主要检测技术及其在相关疾病中的应用。
