Institute of Problems of Chemical Physics, RAS, Academician Semenov avenue 1, Chernogolovka 142432, Moscow Region, Russia.
Institute of Problems of Chemical Physics, RAS, Academician Semenov avenue 1, Chernogolovka 142432, Moscow Region, Russia; Higher Chemical College, Russian Academy of Sciences, D. I. Mendeleev University of Chemical Technology, Miusskaya 9, 125047 Moscow, Russia.
Biochim Biophys Acta Biomembr. 2018 Aug;1860(8):1537-1543. doi: 10.1016/j.bbamem.2018.05.007. Epub 2018 May 21.
Self-diffusion of water-soluble fullerene derivative (WSFD) C[S(CH)SONa]H in mouse red blood cells (RBC) was characterized by H pulsed field gradient NMR technique. It was found that a fraction of fullerene molecules (~13% of the fullerene derivative added in aqueous RBC suspension) shows a self-diffusion coefficient of (5.5 ± 0.8)·10 m/s, which is matching the coefficient of the lateral diffusion of lipids in the erythrocyte membrane (D = (5.4 ± 0.8)·10 m/s). This experimental finding evidences the absorption of the fullerene derivative by RBC. Fullerene derivative molecules are also absorbed by RBC ghosts and phosphatidylcholine liposomes as manifested in self-diffusion coefficients of (7.9 ± 1.2)·10 m/s and (7.7 ± 1.2)·10 m/s, which are also close to the lateral diffusion coefficients of (6.5 ± 1.0)·10 m/s and (8.5 ± 1.3)·10 m/s, respectively. The obtained results suggest that fullerene derivative molecules are, probably, fixed on the RBC surface. The average residence time of the fullerene derivative molecule on RBC was estimated as 440 ± 70 ms. Thus, the pulsed field gradient NMR was shown to be a versatile technique for investigation of the interactions of the fullerene derivatives with blood cells providing essential information, which can be projected on their behavior in-vivo after intravenous administration while screening as potential drug candidates.
水可溶性富勒烯衍生物（WSFD）C[S(CH)SONa]H 在小鼠红细胞（RBC）中的自扩散通过 H 脉冲场梯度 NMR 技术进行了表征。研究发现，一部分富勒烯分子（约加入到 RBC 悬浮液中的富勒烯衍生物的 13%）表现出的自扩散系数为（5.5±0.8）·10 m/s，这与红细胞膜中脂质的横向扩散系数（D=（5.4±0.8）·10 m/s）相匹配。这一实验发现证明了富勒烯衍生物被 RBC 吸收。富勒烯衍生物分子也被 RBC 血影和磷脂囊泡吸收，自扩散系数分别为（7.9±1.2）·10 m/s 和（7.7±1.2）·10 m/s，这也接近横向扩散系数（6.5±1.0）·10 m/s 和（8.5±1.3）·10 m/s。所得结果表明，富勒烯衍生物分子可能固定在 RBC 表面上。富勒烯衍生物分子在 RBC 上的平均停留时间估计为 440±70 ms。因此，脉冲场梯度 NMR 被证明是一种用于研究富勒烯衍生物与血细胞相互作用的多功能技术，提供了重要信息，这些信息可以在静脉注射后预测其在体内的行为，同时筛选作为潜在的药物候选物。
