使用H脉冲场梯度核磁共振研究生物细胞中的水和分子交换。

Water and Molecular Exchange in Biological Cells Studied Using H Pulsed Field Gradient NMR.

作者信息

Avilova Irina A, Volkov Vitaly I

机构信息

Federal Research Center of Problems of Chemical Physics and Medicinal Chemistry RAS, 142432 Chernogolovka, Russia.

Scientific Center in Chernogolovka, The Institute of Solid State Physics RAS, 142432 Chernogolovka, Russia.

出版信息

Membranes (Basel). 2023 May 30;13(6):567. doi: 10.3390/membranes13060567.

DOI:10.3390/membranes13060567

PMID:37367771

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10304187/

Abstract

This review presents the results of studies of molecular exchange processes in various biological systems (erythrocytes, yeast, liposomes, etc.) performed using pulsed field gradient NMR (PFG NMR). The main theory of processing necessary for the analysis of experimental data is briefly presented: the extraction of self-diffusion coefficients, calculation of cell sizes, and permeability of cell membranes. Attention is paid to the results of assessing the permeability of biological membranes for water molecules and biologically active compounds. The results for other systems are also presented: yeast, chlorella, and plant cells. The results of studies of the lateral diffusion of lipid and cholesterol molecules in model bilayers are also presented.

摘要

本综述介绍了使用脉冲场梯度核磁共振（PFG NMR）对各种生物系统（红细胞、酵母、脂质体等）中的分子交换过程进行研究的结果。简要介绍了分析实验数据所需的主要处理理论：自扩散系数的提取、细胞大小的计算以及细胞膜的通透性。重点关注了对生物膜对水分子和生物活性化合物通透性评估的结果。还介绍了其他系统（酵母、小球藻和植物细胞）的研究结果。同时也介绍了模型双层膜中脂质和胆固醇分子横向扩散的研究结果。