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嗜热栖热放线菌菌株NJ4的基因组序列草图，该菌株能够通过整合生物加工从菊粉中生产丁醇。

The Draft Genome Sequence of Clostridium sp. Strain NJ4, a Bacterium Capable of Producing Butanol from Inulin Through Consolidated Bioprocessing.

作者信息

Jiang Yujia, Lu Jiasheng, Chen Tianpeng, Yan Wei, Dong Weiliang, Zhou Jie, Zhang Wenming, Ma Jiangfeng, Jiang Min, Xin Fengxue

机构信息

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Puzhu South Road 30#, Nanjing, 211800, People's Republic of China.

Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing, 211800, People's Republic of China.

出版信息

Curr Microbiol. 2018 Sep;75(9):1221-1225. doi: 10.1007/s00284-018-1513-1. Epub 2018 May 23.

DOI:10.1007/s00284-018-1513-1
PMID:29796852
Abstract

A novel butanogenic Clostridium sp. NJ4 was successfully isolated and characterized, which could directly produce relatively high titer of butanol from inulin through consolidated bioprocessing (CBP). The assembled draft genome of strain NJ4 is 4.09 Mp, containing 3891 encoded protein sequences with G+C content of 30.73%. Among these annotated genes, a levanase, a hypothetical inulinase, and two bifunctional alcohol/aldehyde dehydrogenases (AdhE) were found to play key roles in the achievement of ABE production from inulin through CBP.

摘要

一株新型产丁醇梭菌NJ4被成功分离并鉴定，该菌株可通过整合生物加工（CBP）从菊粉直接生产相对高滴度的丁醇。菌株NJ4的组装草图基因组为4.09Mp，包含3891个编码蛋白质序列，G+C含量为30.73%。在这些注释基因中，发现一种果聚糖酶、一种假定的菊粉酶和两种双功能醇/醛脱氢酶（AdhE）在通过CBP从菊粉生产ABE的过程中起关键作用。

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