State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, China; Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, China.
School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, China; Department of Microbial Pathogenesis, University of Maryland, Baltimore, 21201, USA; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou, 510640, China.
Microb Pathog. 2018 Aug;121:232-237. doi: 10.1016/j.micpath.2018.05.033. Epub 2018 May 23.
Cronobacter species are Gram-negative opportunistic foodborne pathogens that may cause enterocolitis, bacteremia and meningitis in neonates and premature neonates. Lipopolysaccharide (LPS) serves as the major component of the outer membrane of cell, is a potential virulence factor for Cronobacter.
Given the potential importance of this molecule in infection and virulence, SDS-PAGE of LPS, MS and TLC characterization of phospholipids and phenotypic characterization of Cronobacter spp. strains were carried out.
The phospholipids from Cronobacter yielded four major peaks at m/z 719.9, 733.9, 747.9 and 773.9 in the spectrum. All Cronobacter showed O-antigen bands except C. muytjensii ATCC 51329. When Cronobacter defect O-antigen, the outer membrane permeability and cell surface hydrophobicities are increased. All Cronobacter are able to grow under pH 5.0 condition and able to grow under 6% NaCl concentration. C. dublinensis DSM 18705 has a higher infection rate to Caco-2 cells than other Cronobacter.
Invasion of pathogens into a host cell is critical component to an infectious case. And C. dublinensis DSM 18705 has a higher infection rate to Caco-2 cells than other Cronobacter.
克罗诺杆菌属是革兰氏阴性机会致病菌，可引起新生儿和早产儿的肠炎、菌血症和脑膜炎。脂多糖（LPS）是细胞外膜的主要成分，是克罗诺杆菌属的潜在毒力因子。
鉴于该分子在感染和毒力方面的潜在重要性，对 LPS 进行 SDS-PAGE、MS 和 TLC 磷脂特征分析以及克罗诺杆菌属菌株的表型特征分析。
克罗诺杆菌属的磷脂在谱中产生四个主要峰，在 m/z 719.9、733.9、747.9 和 773.9 处。除 C. muytjensii ATCC 51329 外，所有克罗诺杆菌属均显示 O-抗原带。当克罗诺杆菌属缺失 O-抗原时，外膜通透性和细胞表面疏水性增加。所有克罗诺杆菌属都能在 pH 5.0 条件下生长，并能在 6%NaCl 浓度下生长。C. dublinensis DSM 18705 对 Caco-2 细胞的感染率高于其他克罗诺杆菌属。
病原体侵入宿主细胞是感染病例的关键组成部分。C. dublinensis DSM 18705 对 Caco-2 细胞的感染率高于其他克罗诺杆菌属。
