Interdisciplinary Nanoscience Center, Aarhus University, Aarhus 8000 C, Denmark.
Department of Molecular Biology and Genetics, Aarhus University, Aarhus 8000 C, Denmark.
Biotechnol J. 2019 Jan;14(1):e1700634. doi: 10.1002/biot.201700634. Epub 2018 Jun 13.
The fields of DNA and RNA nanotechnology have established nucleic acids as valuable building blocks for functional nanodevices with applications in nanomedicine. Here, a simple method for designing and assembling a 3D scaffolded RNA origami wireframe structure with intrinsic functioning small interfering RNAs (siRNAs) embedded is introduced. Uniquely, the method uses an mRNA fragment as scaffold strand, which is folded by sequence-complementarity of nine shorter synthetic strands. High-yield production of the intended 3D structure is verified by transmission electron microscopy (TEM). Production of functional siRNAs is facilitated by incorporating recognition sites for Dicer at selected locations in the structure, and efficient silencing of a target reporter gene is demonstrated.
DNA 和 RNA 纳米技术领域已经将核酸确立为功能纳米器件的有价值的构建块，这些纳米器件在纳米医学中有应用。在这里，介绍了一种设计和组装具有内在功能的小干扰 RNA（siRNA）嵌入式 3D 支架 RNA 折纸线框结构的简单方法。独特的是，该方法使用 mRNA 片段作为支架链，该支架链通过九根较短的合成链的序列互补折叠而成。通过透射电子显微镜（TEM）验证了预期 3D 结构的高产率生产。通过在结构中的选定位置掺入针对 Dicer 的识别位点，促进了功能性 siRNA 的产生，并证明了对靶标报告基因的有效沉默。
