State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China; Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing, 211198, China; School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 211198, China.
Qinghai Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810008, Qinghai Province, China; Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810008, China.
Biomed Pharmacother. 2018 Aug;104:679-685. doi: 10.1016/j.biopha.2018.05.081. Epub 2018 May 25.
Non-alcoholic fatty liver disease (NAFLD) is the most common liver disease with a broad spectrum of liver injury. Oxidant stress is believed to be the pathogenesis of NAFLD as the "second hit". Hydrogen peroxide is widely used as an oxidant reagent to induce the oxidant injury of cells and larval zebrafish. Recently, cichoric acid is being studied extensively for its obesity attenuating, hepatic steatosis reduction and anti-oxidant effects. In this study, to identify whether CRA could protect the HO induced oxidant injury via anti-oxidant impact by using L02 and HepG2 hepatocytes as in vitro and larval zebrafish as in vivo injury models, and evaluated the protective and anti-oxidant effects of CRA by pretreated it on both in vitro and in vivo models. CRA was found to reduce the production of ROS and MDA, activate the anti-oxidant enzymes SOD and GSH-px, and pathways Keap1-Nrf2 and HO-1. These results demonstrated that CRA might protect the liver injury by its anti-oxidant effect, which could be a potential therapeutic agent of NAFLD.
非酒精性脂肪性肝病（NAFLD）是最常见的肝脏疾病，具有广泛的肝脏损伤谱。氧化应激被认为是 NAFLD 的发病机制，即“二次打击”。过氧化氢被广泛用作诱导细胞和幼鱼氧化损伤的氧化剂。最近，菊苣酸因其减轻肥胖、减少肝脂肪变性和抗氧化作用而受到广泛研究。在这项研究中，为了确定 CRA 是否可以通过抗氧化作用来保护 HO 诱导的氧化损伤，我们使用 L02 和 HepG2 肝细胞作为体外和幼鱼斑马鱼作为体内损伤模型，并通过预先处理体外和体内模型来评估 CRA 的保护和抗氧化作用。结果表明，CRA 可以减少 ROS 和 MDA 的产生，激活抗氧化酶 SOD 和 GSH-px，以及 Keap1-Nrf2 和 HO-1 途径。这些结果表明，CRA 可能通过其抗氧化作用来保护肝脏损伤，这可能是治疗 NAFLD 的潜在药物。
