Rathore K, Joseph B, Sharma D K, Gaurav A, Sharma S K, Milind M, Patel P, Prakash C, Singh L
Department of Veterinary Microbiology, College of Veterinary and Animal Science, Navania, Udaipur, 313601, Rajasthan, India.
Department of Veterinary Public Health, College of Veterinary and Animal Science, Navania, Udaipur, 313601, Rajasthan, India.
Vet World. 2018 Apr;11(4):474-479. doi: 10.14202/vetworld.2018.474-479. Epub 2018 Apr 13.
This study was designed to evaluate the potential of the use of multiplex polymerase chain reaction (PCR) as an alternative to conventional antibiotic sensitivity test.
Isolates of (total = 36) from clinical cases presented to Teaching Veterinary Clinical Complex of College of Veterinary and Animal Sciences (CVAS), Navania, Udaipur, were characterized by morphological, cultural, and biochemical methods. Then, the isolates were further subjected to molecular characterization by PCR targeting -specific sequence (107 bp). Phenotypic antibiotic sensitivity pattern was analyzed by Kirby-Bauer disc diffusion method against 11 commonly used antibiotics in veterinary medicine in and around Udaipur region. The genotypic antibiotic sensitivity pattern was studied against methicillin, aminoglycosides, and tetracycline targeting the gene , -, and by multiplex PCR.
There was 100% correlation between the phenotype and genotype of aminoglycoside resistance, more than 90% correlation for methicillin resistance, and 58.3% in the case tetracycline resistance.
As there is a good correlation between phenotype and genotype of antibiotic resistance, multiplex PCR can be used as an alternative to the conventional antibiotic susceptibility testing, as it can give a rapid and true prediction of antibiotic sensitivity pattern.
本研究旨在评估使用多重聚合酶链反应（PCR）作为传统抗生素敏感性试验替代方法的潜力。
从提交给乌代布尔纳瓦尼亚兽医与动物科学学院（CVAS）教学兽医临床中心的临床病例中分离出的菌株（共36株），通过形态学、培养和生化方法进行鉴定。然后，通过针对特定序列（107 bp）的PCR对分离株进行进一步的分子鉴定。采用 Kirby-Bauer 纸片扩散法分析分离株对乌代布尔地区及周边常用的11种兽用抗生素的表型抗生素敏感性模式。通过多重PCR针对基因、和研究分离株对甲氧西林、氨基糖苷类和四环素的基因型抗生素敏感性模式。
氨基糖苷类耐药的表型和基因型之间存在100%的相关性，甲氧西林耐药的相关性超过90%，四环素耐药的相关性为58.3%。
由于抗生素耐药的表型和基因型之间存在良好的相关性，多重PCR可作为传统抗生素敏感性试验的替代方法，因为它可以快速、准确地预测抗生素敏感性模式。
