Peng M L, Li S N, He Q Q, Zhao J L, Li L L, Ma H T
Key Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
J Anim Physiol Anim Nutr (Berl). 2018 Oct;102(5):1210-1219. doi: 10.1111/jpn.12925. Epub 2018 May 28.
Metabolic disorder is a major health problem and is associated with a number of metabolic diseases. Due to native hyperglycaemia and resistance to exogenous insulin, chickens as a model had used in the studies of adipose tissue biology, metabolism and obesity. But no detailed information is available about the comprehensive changes of serum metabolites at different stages of chicken embryonic development. This study employed LC/MS-QTOF to determine the changes of major functional metabolites at incubation day 14 (E14d), 19 (E19d) and hatching day 1 (H1d), and the associated pathways of differential metabolites during chicken embryonic development were analysed using Metabolite Set Enrichment Analysis method. Results showed that 39 metabolites were significantly changed from E14d to E19d and 68 metabolites were significantly altered from E19d to H1d in chicken embryos. Protein synthesis was promoted by increasing the concentrations of L-glutamine and threonine, and gonadal development was promoted through increasing oestrone content from E14d to E19d in chicken embryos, which indicated that serum glutamine, threonine and oestrone contents may be considered as the candidate indicators for assessment of early embryonic development. 2-oxoglutaric acid mainly contributed to enhancing the citric cycle, and it plays an important role in improving the growth of chicken embryos at the late development; the decreasing of L-glutamine, L-isoleucine and L-leucine contents from E19d to H1d in chicken embryonic development implied their possible functions as the feed additive during early posthatch period of broiler chickens to satisfy the growth. These results provided insights into understand the roles of serum metabolites at different developmental stages of chicken embryos, it also provides available information for chicken as a model to study metabolic disease or human obesity.
代谢紊乱是一个主要的健康问题，与多种代谢性疾病相关。由于鸡存在天然高血糖症以及对外源胰岛素的抵抗性，鸡作为一种模型已被用于脂肪组织生物学、代谢和肥胖症的研究。但是，关于鸡胚胎发育不同阶段血清代谢物的全面变化尚无详细信息。本研究采用液相色谱/质谱联用飞行时间质谱仪（LC/MS-QTOF）来测定孵化第14天（E14d）、第19天（E19d）和出雏第1天（H1d）主要功能代谢物的变化，并使用代谢物集富集分析方法分析鸡胚胎发育过程中差异代谢物的相关途径。结果显示，鸡胚胎中从E14d到E19d有39种代谢物发生显著变化，从E19d到H1d有68种代谢物发生显著改变。鸡胚胎中L-谷氨酰胺和苏氨酸浓度的增加促进了蛋白质合成，从E14d到E19d雌酮含量的增加促进了性腺发育，这表明血清谷氨酰胺、苏氨酸和雌酮含量可被视为评估早期胚胎发育的候选指标。2-氧代戊二酸主要有助于增强柠檬酸循环，并且在促进鸡胚胎后期发育生长中发挥重要作用；鸡胚胎发育过程中从E19d到H1d，L-谷氨酰胺、L-异亮氨酸和L-亮氨酸含量的降低意味着它们可能作为肉仔鸡出雏后早期阶段的饲料添加剂以满足生长需求。这些结果为理解鸡胚胎不同发育阶段血清代谢物的作用提供了见解，也为将鸡作为研究代谢疾病或人类肥胖症的模型提供了有用信息。
