Saleh Tarek M, Ahmed Ebtehal A, Yu Lina, Kwak Ho-Hyun, Hussein Kamal H, Park Kyung-Mee, Kang Byung-Jae, Choi Ki-Young, Kang Kyung-Sun, Woo Heung-Myong
1 Department of Veterinary Science, College of Veterinary Medicine and Stem Cell Institute, Kangwon National University, Chuncheon, Republic of Korea.
2 Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt.
Int J Artif Organs. 2018 Aug;41(8):421-430. doi: 10.1177/0391398818775522. Epub 2018 May 28.
Decellularization of tissues can significantly improve regenerative medicine and tissue engineering by producing natural, less immunogenic, three-dimensional, acellular matrices with high biological activity for transplantation. Decellularized matrices retain specific critical components of native tissues such as stem cell niche, various growth factors, and the ability to regenerate in vivo. However, recellularization and functionalization of these matrices remain limited, highlighting the need to improve the characteristics of decellularized matrices. Incorporating nanoparticles into decellularized tissues can overcome these limitations because nanoparticles possess unique properties such as multifunctionality and can modify the surface of decellularized matrices with additional growth factors, which can be loaded onto the nanoparticles. Therefore, in this minireview, we highlight the various approaches used to improve decellularized matrices with incorporation of nanoparticles and the challenges present in these applications.
组织去细胞化可通过产生天然、低免疫原性、具有高生物活性的三维无细胞基质用于移植，从而显著改善再生医学和组织工程。去细胞化基质保留了天然组织的特定关键成分，如干细胞生态位、各种生长因子以及体内再生能力。然而，这些基质的再细胞化和功能化仍然有限，这凸显了改善去细胞化基质特性的必要性。将纳米颗粒掺入去细胞化组织可以克服这些限制，因为纳米颗粒具有多功能等独特性质，并且可以用额外的生长因子修饰去细胞化基质的表面，这些生长因子可以负载到纳米颗粒上。因此，在这篇综述中，我们重点介绍了通过掺入纳米颗粒来改善去细胞化基质的各种方法以及这些应用中存在的挑战。
