Gupta Sweta K, Mishra Narayan C, Dhasmana Archna
Department of Polymer and Process Engineering, Indian Institute of Technology, Roorkee, 247001, India.
Department of Chemical Engineering, University of Rhode Island, Kingston, RI, 02881, USA.
Methods Mol Biol. 2018;1577:1-10. doi: 10.1007/7651_2017_34.
Decellularization is the process of removal of native cells from tissue, leaving behind a three-dimensional (3D) ultrastructure of extracellular matrix (ECM) proteins while preserving the bioactivity and mechanics of the tissue. It offers a unique top-down approach for fabricating ECM based natural scaffold for tissue engineering application. Herein, this chapter presents the fabrication of decellularized scaffold employing different methods: whole organ perfusion, immersion and agitation, pressure gradient, and supercritical fluid. The decellularized scaffold aims to exploit the nature-designed 3D architecture, a successful platform technology, for creating scaffolding materials for tissue engineering and regenerative medicine.
去细胞化是从组织中去除天然细胞的过程，留下细胞外基质（ECM）蛋白的三维（3D）超微结构，同时保留组织的生物活性和力学性能。它为制造用于组织工程应用的基于ECM的天然支架提供了一种独特的自上而下的方法。本章介绍了采用不同方法制备去细胞支架的过程：全器官灌注、浸泡搅拌、压力梯度和超临界流体。去细胞支架旨在利用自然设计的3D结构这一成功的平台技术，来创建用于组织工程和再生医学的支架材料。
