大肠杆菌亮氨酸tRNA家族：supP(Am)抑制基因的核苷酸序列。
Leucine tRNA family of Escherichia coli: nucleotide sequence of the supP(Am) suppressor gene.
作者信息
Thorbjarnardóttir S, Dingermann T, Rafnar T, Andrésson O S, Söll D, Eggertsson G
出版信息
J Bacteriol. 1985 Jan;161(1):219-22. doi: 10.1128/jb.161.1.219-222.1985.
Abstract
We describe the cloning and the DNA sequence of an amber suppressor allele of the Escherichia coli leuX (supP) gene. The suppressor allele codes for a tRNA with anticodon CUA, presumably derived by a single base change from a CAA anticodon. The mature coding sequence of the leuX gene is preceded by a putative Pribnow box sequence (TATAAT) and followed by a termination signal. The sequence of the leuX-coded tRNA is compared with the sequences of the four remaining tRNALeu isoacceptors of E. coli and with two tRNALeu species from bacteriophage T4 and T5. The conserved nucleotides in these seven tRNAs recognized by E. coli leucyl-tRNA synthetase are located mainly in the aminoacyl stem and in the D-stem/loop region.
摘要
我们描述了大肠杆菌leuX（supP）基因琥珀抑制子等位基因的克隆及DNA序列。该抑制子等位基因编码一种反密码子为CUA的tRNA，推测是由反密码子CAA经单个碱基改变而来。leuX基因的成熟编码序列之前有一个假定的普里布诺框序列（TATAAT），之后有一个终止信号。将leuX编码的tRNA序列与大肠杆菌其余四种亮氨酰tRNA同工受体的序列以及来自噬菌体T4和T5的两种亮氨酰tRNA的序列进行了比较。大肠杆菌亮氨酰tRNA合成酶识别的这七种tRNA中的保守核苷酸主要位于氨酰基茎和D茎/环区域。
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