Uemura H, Thorbjarnardóttir S, Gamulin V, Yano J, Andrésson O S, Söll D, Eggertsson G
J Bacteriol. 1985 Sep;163(3):1288-9. doi: 10.1128/jb.163.3.1288-1289.1985.
We describe the cloning and nucleotide sequence of a new tRNALys gene, lysV, in Escherichia coli. An ochre suppressor allele of this gene, supN, codes for a tRNALys with anticodon UUA, presumably derived by a single base change from a wild-type UUU anticodon. The sequence of the supN tRNALys is identical to the sequence of ochre suppressor tRNAs encoded by mutant alleles at the lysT locus. This locus, which contains the two previously known tRNALys genes of E. coli, is located far from the lysV locus on the chromosome.
我们描述了大肠杆菌中一个新的赖氨酸转运RNA基因(lysV)的克隆及核苷酸序列。该基因的一个赭石型抑制等位基因supN编码一种反密码子为UUA的赖氨酸转运RNA,推测是由野生型反密码子UUU经单碱基变化产生的。supN赖氨酸转运RNA的序列与lysT位点突变等位基因编码的赭石型抑制转运RNA的序列相同。该位点包含大肠杆菌先前已知的两个赖氨酸转运RNA基因,位于染色体上远离lysV位点的位置。