Naccache P H, Molski T F, Borgeat P, Sha'afi R I
J Cell Physiol. 1985 Feb;122(2):273-80. doi: 10.1002/jcp.1041220217.
The addition of low concentrations (less than 10(-7) M) of the calcium ionophore A23187 to rabbit neutrophils releases the intracellular pool of calcium previously shown in radioactive steady-state and chlortetracycline fluorescence studies to be mobilized by chemotactic factors. A23187 at these concentrations elicits no functional responses from these cells. However, A23187, added before chemotactic factors such as fMet-Leu-Phe and leukotriene B4, inhibits the ability of the latter stimuli to induce, in the presence of cytochalasin B, an exocytotic release of the neutrophil's cytoplasmic granules. These results imply that the chemotactic-factor-induced release of intracellular calcium is a necessary event for the optimal activation of the neutrophils. Phorbol ester-induced neutrophil degranulation on the other hand is unaffected by exposure to A23187, thereby completely dissociating its mechanism of action from rises in cytoplasmic free calcium.
向兔中性粒细胞中添加低浓度（小于10⁻⁷M）的钙离子载体A23187会释放出细胞内的钙池，先前在放射性稳态和金霉素荧光研究中已表明该钙池会被趋化因子动员。这些浓度的A23187不会引发这些细胞的功能反应。然而，在趋化因子如甲酰甲硫氨酸-亮氨酸-苯丙氨酸和白三烯B4之前添加A23187，会抑制后者在细胞松弛素B存在的情况下诱导中性粒细胞胞质颗粒胞吐释放的能力。这些结果表明，趋化因子诱导的细胞内钙释放是中性粒细胞最佳激活的必要事件。另一方面，佛波酯诱导的中性粒细胞脱颗粒不受暴露于A23187的影响，从而使其作用机制与细胞质游离钙的升高完全分离。
