Naccache P H, Molski T F, Borgeat P, Sha'afi R I
J Cell Physiol. 1984 Jan;118(1):13-8. doi: 10.1002/jcp.1041180104.
The possible involvement of membrane-bound calcium in the mechanism of action of leukotriene B4 was examined using the fluorescent chelate probe, chlortetracycline. Leukotriene B4 was found to cause a rapid release of membrane-bound calcium at physiologically relevant concentrations. This effect of leukotriene B4 is stereospecific and its magnitude is decreased upon the transformation of leukotriene B4 into its omega-hydroxy and omega-carboxy metabolites. The pool of calcium affected by leukotriene B4 appears to be the same as that released by other chemotactic factors such as formyl-methionyl-leucyl-phenylalanine (f-Met-Leu-Phe). Similarly, preincubation with f-Met-Leu-Phe results in a decreased responsiveness of the cells to the addition of leukotriene B4. These results extend further the analogy between the mechanism of action of peptidic and lipid chemotactic factors, and emphasize the central role of the intracellular redistribution of calcium, as inferred and monitored by chlortetracycline fluorescence and steady-state isotopic flux studies, in neutrophil activation.
使用荧光螯合探针金霉素，研究了膜结合钙在白三烯B4作用机制中的可能参与情况。发现白三烯B4在生理相关浓度下可导致膜结合钙的快速释放。白三烯B4的这种作用具有立体特异性，并且在白三烯B4转化为其ω-羟基和ω-羧基代谢产物后，其作用强度会降低。受白三烯B4影响的钙池似乎与其他趋化因子（如甲酰甲硫氨酰亮氨酰苯丙氨酸，f-Met-Leu-Phe）释放的钙池相同。同样，用f-Met-Leu-Phe预孵育会导致细胞对白三烯B4添加的反应性降低。这些结果进一步扩展了肽类和脂质趋化因子作用机制之间的相似性，并强调了钙的细胞内重新分布在中性粒细胞激活中的核心作用，这是通过金霉素荧光和稳态同位素通量研究推断和监测到的。
