Sha'afi R I, Naccache P H, Molski T F, Borgeat P, Goetzl E J
J Cell Physiol. 1981 Sep;108(3):401-8. doi: 10.1002/jcp.1041080314.
We have found that exogenous leukotriene B4 modifies calcium homeostasis in rabbit neutrophils in a manner essentially analogous to that of the chemotactic peptide f-Met-Leu-Phe. Leukotriene B4 causes a rapid and dose-dependent increase in membrane permeability to calcium and a release of calcium from previously unexchangeable intracellular pool(s). The net result of these changes is to transiently elevate the intracellular level of exchangeable calcium. A stereoisomer of leukotriene B4 with greatly reduced secretory activity toward neutrophils (5S, 12S-di HETE) is essentially without effect on the rate of 45Ca uptake at concentrations equal to those that produce near maximal enhancement by leukotriene B4. Leukotriene B4, in addition to its effects on calcium metabolism, also increases the rate of 22Na influx into rabbit neutrophils. The relationships between the action of leukotriene B4 on calcium homeostasis and the neutrophil-directed activities of arachidonic acid and its lipoxygenase metabolites are discussed
我们发现，外源性白三烯B4对兔中性粒细胞钙稳态的影响方式，与趋化肽f-Met-Leu-Phe基本类似。白三烯B4可使细胞膜对钙的通透性迅速增加，且呈剂量依赖性，并促使钙从先前不可交换的细胞内钙库中释放出来。这些变化的最终结果是使可交换钙的细胞内水平短暂升高。对白三烯B4的一种立体异构体而言，其对中性粒细胞的分泌活性大幅降低（5S, 12S-二羟二十碳四烯酸），在与白三烯B4产生接近最大增强作用浓度相同的情况下，对45Ca摄取速率基本无影响。白三烯B4除了对钙代谢有影响外，还会增加22Na流入兔中性粒细胞的速率。本文讨论了白三烯B4对钙稳态的作用与花生四烯酸及其脂氧合酶代谢产物对中性粒细胞的作用之间的关系。
