Showell H J, Naccache P H, Borgeat P, Picard S, Vallerand P, Becker E L, Sha'afi R I
J Immunol. 1982 Feb;128(2):811-6.
We have described in det ail the secretory activity of leukotriene B4 toward rabbit neutrophils. Leukotriene B4 rapidly and vigorously degranulates rabbit neutrophils. This activity is stereospecific, cytochalasin B-dependent, and is enhanced by extracellular calcium. Pretreatment with leukotriene B4 deactivates rabbit neutrophils, i.e., cells so treated do not respond to stimulation by an additional bolus of leukotriene B4. In addition, the secretory activity of leukotriene B4 is sharply dependent on the simultaneous presence of cytochalasin B. Rabbit neutrophils therefore exhibit the previously described desensitization to the effect of cytochalasin B. In these and other discussed respects the characteristics of the leukotriene B4-induced degranulation of rabbit neutrophils are strikingly similar to those of the chemotactic factors. These results support the hypothesis that leukotriene B4 mediates, at least in part, the secretory, and possibly other, activities of chemotactic factors.
我们已经详细描述了白三烯B4对兔中性粒细胞的分泌活性。白三烯B4能迅速且强烈地使兔中性粒细胞脱颗粒。这种活性具有立体特异性，依赖于细胞松弛素B，并且细胞外钙可增强该活性。用白三烯B4预处理会使兔中性粒细胞失活，即经如此处理的细胞对额外注入的白三烯B4刺激无反应。此外，白三烯B4的分泌活性强烈依赖于细胞松弛素B的同时存在。因此，兔中性粒细胞表现出先前描述的对细胞松弛素B作用的脱敏现象。在这些以及其他讨论的方面，白三烯B4诱导兔中性粒细胞脱颗粒的特征与趋化因子的特征极为相似。这些结果支持了这样一种假说，即白三烯B4至少部分介导了趋化因子 的分泌活性以及可能的其他活性。
