Ziola B, Hader W J
J Neuroimmunol. 1985 Feb-Mar;7(5-6):315-30.
Viral antigen-induced blastogenesis of lymphocytes from multiple sclerosis (MS) patients was investigated to determine if the responses were actively suppressed. We found that depletion of adherent cells increased measles and herpes simplex I virus antigen-induced transformation of MS lymphocytes. Addition of indomethacin to cultures of unfractionated MS lymphocytes also caused an increase in viral antigen-induced responses. These two facts, plus finding that the cell type mediating the immunosuppression did not rosette with 2-aminoethylisothiouronium bromide hydrobromide-treated sheep red blood cells, indicate that the suppressed T-cell responsiveness of MS patients is caused by macrophages rather than T-cells. These results have a major implication for the divergent published data on blastogenesis induced in MS patient lymphocytes by specific antigens, viral or otherwise. We feel the inconsistencies may simply have arisen from the different lymphocyte isolation and washing procedures used giving variable levels of macrophages and, hence, variable levels of immune suppression. This clearly suggests that induction of blastogenesis in MS patient lymphocytes by a wider array of infectious agent antigens and by various neural antigens should now be undertaken using adherent cell-depleted lymphocytes.
为了确定多发性硬化症（MS）患者淋巴细胞的病毒抗原诱导的母细胞化反应是否受到主动抑制，我们对其进行了研究。我们发现，去除贴壁细胞可增加麻疹病毒和单纯疱疹病毒I抗原诱导的MS淋巴细胞转化。在未分离的MS淋巴细胞培养物中添加吲哚美辛也会导致病毒抗原诱导的反应增加。这两个事实，再加上发现介导免疫抑制的细胞类型不与氢溴酸2-氨基乙基异硫脲处理的绵羊红细胞形成玫瑰花结，表明MS患者T细胞反应性受到抑制是由巨噬细胞而非T细胞引起的。这些结果对已发表的关于MS患者淋巴细胞由特定抗原（病毒或其他）诱导的母细胞化反应的不同数据具有重要意义。我们认为，这些不一致可能仅仅是由于所使用的淋巴细胞分离和洗涤程序不同，导致巨噬细胞水平不同，从而免疫抑制水平也不同。这清楚地表明，现在应该使用去除贴壁细胞的淋巴细胞，对更广泛的感染因子抗原和各种神经抗原诱导MS患者淋巴细胞的母细胞化反应进行研究。
